Anti-PELI1 antibody (STJA0007462)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human PELI1
General Information
| Short Description | Rabbit polyclonal anti-E3 ubiquitin-protein ligase pellino homolog 1 for use in WB in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PELI1 |
| Gene ID | 57162 |
| Uniprot ID | PELI1_HUMAN |
| Immunogen | Synthesized peptide derived from human PELI1 |
| Specificity | This antibody detects endogenous levels of PELI1 at Human, Mouse |
Additional Info
| Post Translational Modifications | Phosphorylation by IRAK1 and IRAK4 enhances its E3 ligase activity. Phosphorylated by ATM in response to DNA damage, promoting localization to DNA double-strand breaks (DSBs) and ability to mediate 'Lys-63'-linked ubiquitination of NBN. Sumoylated. |
| Function | E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. Acts as a positive regulator of inflammatory response in microglia through activation of NF-kappa-B and MAP kinase. Mediates 'Lys-63'-linked polyubiquitination of IRAK1 allowing subsequent NF-kappa-B activation. Conjugates 'Lys-63'-linked ubiquitin chains to the adapter protein ASC/PYCARD, which in turn is crucial for NLRP3 inflammasome activation. Mediates 'Lys-48'-linked polyubiquitination of RIPK3 leading to its subsequent proteasome-dependent degradation.preferentially recognizes and mediates the degradation of the 'Thr-182' phosphorylated form of RIPK3. Negatively regulates necroptosis by reducing RIPK3 expression. Mediates 'Lys-63'-linked ubiquitination of RIPK1. Following phosphorylation by ATM, catalyzes 'Lys-63'-linked ubiquitination of NBN, promoting DNA repair via homologous recombination. Negatively regulates activation of the metabolic mTORC1 signaling pathway by mediating 'Lys-63'-linked ubiquitination of mTORC1-inhibitory protein TSC1 and thereby promoting TSC1/TSC2 complex stability. |
| Protein Name | E3 Ubiquitin-Protein Ligase Pellino Homolog 1Pellino-1Pellino-Related Intracellular-Signaling MoleculeRing-Type E3 Ubiquitin Transferase Pellino Homolog 1 |
| Database Links | Reactome: R-HSA-5675482Reactome: R-HSA-9020702Reactome: R-HSA-937039Reactome: R-HSA-975144 |
| Cellular Localisation | ChromosomeLocalizes To Dna Double-Strand Breaks (Dsbs) In Response To Dna Damage |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Pellino Homolog 1 antibodyAnti-Pellino-1 antibodyAnti-Pellino-Related Intracellular-Signaling Molecule antibodyAnti-Ring-Type E3 Ubiquitin Transferase Pellino Homolog 1 antibodyAnti-PELI1 antibodyAnti-PRISM antibody |
Information sourced from Uniprot.org