• Western blot analysis of lysates from Hela cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-PEF1 antibody (109-159 aa) (STJ195013)

SKU:
STJ195013

Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Peflin (109-159 aa) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC-P 1:50-300
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PEF1
Gene ID: 553115
Uniprot ID: PEF1_HUMAN
Immunogen Region: 109-159 aa
Specificity: This antibody detects endogenous levels of PEF1 at Human/Mouse/Rat
Immunogen: Synthesized peptide derived from the human PEF1 at the amino acid range 109-159
Function Calcium-binding protein that acts as an adapter that bridges unrelated proteins or stabilizes weak protein-protein complexes in response to calcium. Together with PDCD6, acts as calcium-dependent adapter for the BCR(KLHL12) complex, a complex involved in endoplasmic reticulum (ER)-Golgi transport by regulating the size of COPII coats. In response to cytosolic calcium increase, the heterodimer formed with PDCD6 interacts with, and bridges together the BCR(KLHL12) complex and SEC31 (SEC31A or SEC31B), promoting monoubiquitination of SEC31 and subsequent collagen export, which is required for neural crest specification. Its role in the heterodimer formed with PDCD6 is however unclear: some evidence shows that PEF1 and PDCD6 work together and promote association between PDCD6 and SEC31 in presence of calcium. Other reports show that PEF1 dissociates from PDCD6 in presence of calcium, and may act as a negative regulator of PDCD6. Also acts as a negative regulator of ER-Golgi transport.possibly by inhibiting interaction between PDCD6 and SEC31.
Protein Name Peflin
Pef Protein With A Long N-Terminal Hydrophobic Domain
Penta-Ef Hand Domain-Containing Protein 1
Cellular Localisation Cytoplasm
Endoplasmic Reticulum
Membrane
Peripheral Membrane Protein
Cytoplasmic Vesicle
Copii-Coated Vesicle Membrane
Membrane-Associated In The Presence Of Ca(2+)
Localizes To Endoplasmic Reticulum Exit Site (Eres)
Alternative Antibody Names Anti-Peflin antibody
Anti-Pef Protein With A Long N-Terminal Hydrophobic Domain antibody
Anti-Penta-Ef Hand Domain-Containing Protein 1 antibody
Anti-PEF1 antibody
Anti-ABP32 antibody
Anti-UNQ1845 antibody
Anti-PRO3573 antibody

Information sourced from Uniprot.org

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