| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 IHC-P:1:50-1:200 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PDXP |
| Gene ID: | 57026 |
| Uniprot ID: | PLPP_HUMAN |
| Immunogen Region: | C-Term |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human PDXP (NP_064711.1). |
| Immunogen Sequence: | VETASGRQALVVGKPSPYMF ECITENFSIDPARTLMVGDR LETDILFGHRCGMTTVLTLT GVSRLEEAQAYLAAGQHDLV PHYYVESIADLTEGLED |
| Tissue Specificity | Ubiquitously expressed (at protein level). Highly expressed in all the regions of central nerve system except the spinal cord. Also expressed at high level in liver and testis. In fetus, it is weakly expressed in all organs except brain. |
| Function | Functions as a pyridoxal phosphate (PLP) phosphatase, which also catalyzes the dephosphorylation of pyridoxine 5'-phosphate (PNP) and pyridoxamine 5'-phosphate (PMP), with order of substrate preference PLP > PNP > PMP and therefore plays a role in vitamin B6 metabolism. Also functions as a protein serine phosphatase that specifically dephosphorylates 'Ser-3' in proteins of the actin-depolymerizing factor (ADF)/cofilin family like CFL1 and DSTN. Thereby, regulates cofilin-dependent actin cytoskeleton reorganization, being required for normal progress through mitosis and normal cytokinesis. Does not dephosphorylate phosphothreonines in LIMK1. Does not dephosphorylate peptides containing phosphotyrosine. |
| Protein Name | Chronophin Pyridoxal Phosphate Phosphatase Plp Phosphatase |
| Cellular Localisation | Cytoplasm Cytosol Cytoskeleton Cell Projection Ruffle Membrane Peripheral Membrane Protein Cytoplasmic Side Lamellipodium Membrane Cell Membrane Colocalizes With The Actin Cytoskeleton In Membrane Ruffles And Lamellipodia Diffusely Distributed Throughout The Cytosol During Pro-Metaphase And Metaphase Detected At The Dynamic Cell Poles During Telophase Detected At The Cleavage Furrow And Contractile Ring During Cytokinesis Transiently Detected At The Plasma Membrane In Late Stages Of Cytokinesis Detected At The Midbody |
| Alternative Antibody Names | Anti-Chronophin antibody Anti-Pyridoxal Phosphate Phosphatase antibody Anti-Plp Phosphatase antibody Anti-PDXP antibody Anti-CIN antibody Anti-PLP antibody Anti-PLPP antibody |
Information sourced from Uniprot.org
