• Western blot analysis of HeLa, using PDLIM5 Rabbit polyclonal antibody (STJ11104657) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PDLIM5 antibody (300-400) (STJ11104657)

SKU:
STJ11104657

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Pdz And Lim Domain Protein 5 (300-400) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PDLIM5
Gene ID: 10611
Uniprot ID: PDLI5_HUMAN
Immunogen Region: 300-400
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 300-400 of human PDLIM5 (NP_006448.5).
Immunogen Sequence: ADNTKKANNSQEPSPQLASS VASTRSMPESLDSPTSGRPG VTSLTAAAAFKPVGSTGVIK SPSWQRPNQGVPSTGRISNS ATYSGSVAPANSALGQTQPS D
Tissue Specificity Heart and skeletal muscle specific. Expression is commonly increased in the brain of patients with bipolar disorder, schizophrenia, and major depression.
Function May play an important role in the heart development by scaffolding PKC to the Z-disk region. May play a role in the regulation of cardiomyocyte expansion. Isoforms lacking the LIM domains may negatively modulate the scaffolding activity of isoform 1. Overexpression promotes the development of heart hypertrophy. Contributes to the regulation of dendritic spine morphogenesis in neurons. May be required to restrain postsynaptic growth of excitatory synapses. Isoform 1, but not isoform 2, expression favors spine thinning and elongation.
Protein Name Pdz And Lim Domain Protein 5
Enigma Homolog
Enigma-Like Pdz And Lim Domains Protein
Database Links Reactome: R-HSA-6794361
Cellular Localisation Postsynaptic Density
Presynapse
Postsynapse
Cytoplasm
Cytosol
Detected Both At Presynaptic And Postsynaptic Sites
Exclusively At Excitatory Synapses
But Not Inhibitory Synapses
In Hippocampal Neurons
Alternative Antibody Names Anti-Pdz And Lim Domain Protein 5 antibody
Anti-Enigma Homolog antibody
Anti-Enigma-Like Pdz And Lim Domains Protein antibody
Anti-PDLIM5 antibody
Anti-ENH antibody
Anti-L9 antibody

Information sourced from Uniprot.org

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