Biotinylated STJ72559 (0. 03ยตg/ml) staining of Rat Pancreas lysate (35ยตg protein in RIPA buffer) , exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-PDIA2, Biotinylated antibody (477-481) {Biotin} (STJ73242)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationBiotin
IsotypeIgG
STJ73242-100
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General Information

Short DescriptionGoat polyclonal antibody anti-PDIA2, Biotinylated (477-481) is suitable for use in ELISA and Western Blot research applications.
ApplicationsPep-ELISA/WB
HostGoat
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationBiotin
Concentration0.5 mg/mL
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution RangePeptide ELISA: antibody detection limit dilution 1:64000.
WB: Approx 65kDa band observed in Rat Pancreas lysates (calculated MW of 58.5kDa according to Rat NP_001099245.2). See non-biotinylated parental product's datasheet for further QC data. Re
Formulation0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Storage InstructionStore at-20ยฐC on receipt and minimise freeze-thaw cycles.

Target Information

Gene SymbolPDIA2
Gene ID64714
Uniprot IDPDIA2_HUMAN
Accession NumberNP_006840.2
Immunogen Region477-481
Immunogen SequenceEYKSTRDLETFSK.

Additional Info

Post Translational Modifications The disulfide-linked homodimer exhibits an enhanced chaperone activity. Glycosylated.
Function Acts as an intracellular estrogen-binding protein. May be involved in modulating cellular levels and biological functions of estrogens in the pancreas. May act as a chaperone that inhibits aggregation of misfolded proteins.
Protein Name Protein Disulfide-Isomerase A2
Pancreas-Specific Protein Disulfide Isomerase
Pdip
Cellular Localisation Endoplasmic Reticulum Lumen
Alternative Antibody Names Anti-Protein Disulfide-Isomerase A2 antibody
Anti-Pancreas-Specific Protein Disulfide Isomerase antibody
Anti-Pdip antibody
Anti-PDIA2 antibody
Anti-PDIP antibody

Information sourced from Uniprot.org

Citations

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