• Immunohistochemistry analysis of PDHB in paraffin-embedded rat kidney tissue using PDHB Rabbit monoclonal antibody (STJ11102123) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of PDHB in paraffin-embedded mouse kidney tissue using PDHB Rabbit monoclonal antibody (STJ11102123) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of PDHB in paraffin-embedded human colon carcinoma tissue using PDHB Rabbit monoclonal antibody (STJ11102123) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of PDHB in paraffin-embedded human lung cancer tissue using PDHB Rabbit monoclonal antibody (STJ11102123) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of PDHB in paraffin-embedded human liver cancer tissue using PDHB Rabbit monoclonal antibody (STJ11102123) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of PDHB in paraffin-embedded human thyroid cancer tissue using PDHB Rabbit monoclonal antibody (STJ11102123) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using Pyruvate Dehydrogenase E1 beta subunit Rabbit monoclonal antibody (STJ11102123) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PDHB antibody (260-359) [S3MR] (STJ11102123)

SKU:
STJ11102123

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-PDHB (260-359) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PDHB
Gene ID: 5162
Uniprot ID: ODPB_HUMAN
Immunogen Region: 260-359
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 260-359 of human Pyruvate Dehydrogenase E1 beta subunit (P11177).
Immunogen Sequence: GVECEVINMRTIRPMDMETI EASVMKTNHLVTVEGGWPQF GVGAEICARIMEGPAFNFLD APAVRVTGADVPMPYAKILE DNSIPQVKDIIFAIKKTLNI
Function The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.
Protein Name Pyruvate Dehydrogenase E1 Component Subunit Beta - Mitochondrial
Pdhe1-B
Database Links Reactome: R-HSA-204174
Reactome: R-HSA-389661
Reactome: R-HSA-5362517
Reactome: R-HSA-70268
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Pyruvate Dehydrogenase E1 Component Subunit Beta - Mitochondrial antibody
Anti-Pdhe1-B antibody
Anti-PDHB antibody
Anti-PHE1B antibody

Information sourced from Uniprot.org

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