| Host: | Rabbit |
| Applications: | WB/IHC-P/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | S3MR |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PDHB |
| Gene ID: | 5162 |
| Uniprot ID: | ODPB_HUMAN |
| Immunogen Region: | 260-359 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 260-359 of human Pyruvate Dehydrogenase E1 beta subunit (P11177). |
| Immunogen Sequence: | GVECEVINMRTIRPMDMETI EASVMKTNHLVTVEGGWPQF GVGAEICARIMEGPAFNFLD APAVRVTGADVPMPYAKILE DNSIPQVKDIIFAIKKTLNI |
| Function | The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle. |
| Protein Name | Pyruvate Dehydrogenase E1 Component Subunit Beta - MitochondrialPdhe1-B |
| Database Links | Reactome: R-HSA-204174Reactome: R-HSA-5362517Reactome: R-HSA-9837999Reactome: R-HSA-9861559 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Antibody Names | Anti-Pyruvate Dehydrogenase E1 Component Subunit Beta - Mitochondrial antibodyAnti-Pdhe1-B antibodyAnti-PDHB antibodyAnti-PHE1B antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and NANS knockout (KO) 293T cells, using [KO Validated] NANS Rabbit polyclonal antibody (STJ11100751) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89689/358177/STJ11100751_1__56006.1713122486.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and NANS knockout (KO) 293T cells, using [KO Validated] NANS Rabbit polyclonal antibody (STJ11100751) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunofluorescence analysis of MCF7 cells using [KD Validated] SCD Rabbit polyclonal antibody (STJ11105577) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/178221/370505/STJ11105577_1__69759.1713136460.jpg?c=1 "Immunofluorescence analysis of MCF7 cells using [KD Validated] SCD Rabbit polyclonal antibody (STJ11105577) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
