• Immunohistochemistry analysis of paraffin-embedded mouse retina using PDE6B antibody (STJ29022) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat retina using PDE6B antibody (STJ29022) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using PDE6B antibody (STJ29022) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PDE6B antibody (1-190) (STJ29022)

SKU:
STJ29022

$151.88 - $705.38
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PDE6B (1-190) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PDE6B
Gene ID: 5158
Uniprot ID: PDE6B_HUMAN
Immunogen Region: 1-190
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human PDE6B (NP_001138763.1).
Immunogen Sequence: MSLSEEQARSFLDQNPDFAR QYFGKKLSPENVAAACEDGC PPDCDSLRDLCQVEESTALL ELVQDMQESINMERVVFKVL RRLCTLLQADRCSLFMYRQR NGVAELATRLFSVQPDSVLE DCLVPPDSEIVFPLDIGVVG HVAQTKKMVNVEDVAECPHF SSFADELTDYKTKNMLATPI MNGKDVVAVI
Function Rod-specific cGMP phosphodiesterase that catalyzes the hydrolysis of 3',5'-cyclic GMP. Necessary for the formation of a functional phosphodiesterase holoenzyme. Involved in retinal circadian rhythm photoentrainment via modulation of UVA and orange light-induced phase-shift of the retina clock. May participate in processes of transmission and amplification of the visual signal.
Protein Name Rod Cgmp-Specific 3' -5'-Cyclic Phosphodiesterase Subunit Beta
Gmp-Pde Beta
Database Links Reactome: R-HSA-2485179
Reactome: R-HSA-2514859
Reactome: R-HSA-4086398
Cellular Localisation Membrane
Lipid-Anchor
Cell Projection
Cilium
Photoreceptor Outer Segment
Alternative Antibody Names Anti-Rod Cgmp-Specific 3' -5'-Cyclic Phosphodiesterase Subunit Beta antibody
Anti-Gmp-Pde Beta antibody
Anti-PDE6B antibody
Anti-PDEB antibody

Information sourced from Uniprot.org

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