Anti-PDE4D antibody (N-Term) {FITC} (STJ502339)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationFITC
IsotypeIgG
ImmunogenA 20 amino acid synthetic peptide taken from the N-terminus corresopnding to the PDE4D5 variant. A This antibody does not cross-react with other members of the PDE4D family or with other PDE4 proteins (PDE4A, PDE4B and PDE4C) or with other PDE family
General Information
| Short Description | Rabbit polyclonal antibody anti-PDE4D (N-Term) is suitable for use in Confocal Microscopy, ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation and Western Blot research applications. |
| Applications | CM/ELISA/ICC/IF/IHC/IP/WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | FITC |
| Concentration | 0.65 ยตg/ยตl |
| Purification | Affinity Purified |
| Dilution Range | WB: 1:500DB: 1:10, 000ELISA: 1:10, 000IP: 1:150IHC: 1:200ICC: 1:200IF: 1:200CFM: 1:200 |
| Formulation | Contains Tris, HCl/Glycine buffer pH 7.4-7.8, 30% Glycerol and 0.5% BSA, along with cryo-protective agents, Hepes, and long-term preservatives (0.02% Sodium Azide). |
| Storage Instruction | Store at-20ยฐC for long term storage. Avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | PDE4D |
| Gene ID | 5144 |
| Uniprot ID | PDE4D_HUMAN |
| Immunogen | A 20 amino acid synthetic peptide taken from the N-terminus corresopnding to the PDE4D5 variant. A This antibody does not cross-react with other members of the PDE4D family or with other PDE4 proteins (PDE4A, PDE4B and PDE4C) or with other PDE family |
| Immunogen Region | N-Term |
Additional Info
| Tissue Specificity | Expressed in colonic epithelial cells (at protein level). Widespread.most abundant in skeletal muscle. Isoform 6: Detected in brain. Isoform 8: Detected in brain, placenta, lung and kidney. Isoform 7: Detected in heart and skeletal muscle. |
| Post Translational Modifications | Long isoforms that share a conserved PKA phosphorylation site in the N-terminus are activated by PKA through phosphorylation. Isoform 3 and isoform 7 are activated by phosphorylation (in vitro), but not isoform 6. Isoform N3 and isoform 12 are phosphorylated on Ser-49, Ser-51, Ser-55 and Ser-59. Sumoylation of long isoforms by PIAS4 augments their activation by PKA phosphorylation and represses their inhibition by ERK phosphorylation. |
| Function | Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. |
| Protein Name | 3' -5'-Cyclic-Amp Phosphodiesterase 4dDpde3Pde43Camp-Specific Phosphodiesterase 4d |
| Database Links | Reactome: R-HSA-180024Reactome: R-HSA-418555 |
| Cellular Localisation | Apical Cell MembraneCytoplasmMembraneCytoskeletonMicrotubule Organizing CenterCentrosomeFound In The Soluble FractionAssociated With MembranesAnd Associated With The Cytoskeleton And The CentrosomeColocalized With Shank2 To The Apical Membrane Of Colonic Crypt Cells |
| Alternative Antibody Names | Anti-3' -5'-Cyclic-Amp Phosphodiesterase 4d antibodyAnti-Dpde3 antibodyAnti-Pde43 antibodyAnti-Camp-Specific Phosphodiesterase 4d antibodyAnti-PDE4D antibodyAnti-DPDE3 antibody |
Information sourced from Uniprot.org