• Immunofluorescence of fixed frozen section of rat seminiferous tubule stained with Anti-PDE4A primary antibody (STJ502296). The localization is restricted to a subset of germ cells.

Anti-PDE4A antibody (STJ502296)

SKU:
STJ502296-100

£481.50
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Host: Rabbit
Applications: CM/ELISA/ICC/IF/IHC/IP/WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PDE4A is suitable for use in Confocal Microscopy, ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Purification: Affinity Purified
Concentration: 1.00-1.25 µg/µl
Dilution Range: WB: 1:1, 000
DB: 1:10, 000
ELISA: 1:10, 000
IP: 1:250
IHC: 1:200
ICC: 1:200
IF: 1:200
CFM: 1:200
Storage Instruction: Store at-20°C for long term storage. Avoid freeze-thaw cycles.
Gene Symbol: PDE4A
Gene ID: 5141
Uniprot ID: PDE4A_HUMAN
Immunogen: Synthetic peptide specific only for PDE4A8 protein.
Tissue Specificity Isoform 1: Expressed in lymphoid cell subsets including CD8-positive T cells and T-helper 2 cells. Expressed in dendritic cells. Isoform 2: Highly expressed in liver, stomach, testis, thyroid and adrenal glands and at a lower extent in placenta, kidney, pancreas, ovary, uterus and skin. Expressed in myeloid cell subsets including dendritic cells, monocytes, macrophages, eosinophils and mast cells. Expressed in natural killer cells. Expressed in bronchial smooth muscle. Isoform 6: Expressed at high levels in the heart and small intestine. It is also found in the brain, kidney, spleen, colon, salivary gland, ovary and peripheral blood lymphocytes. Isoform 7: Expressed predominantly in skeletal muscle and brain and at lower levels in the testis. Found in specific neuronal subpopulations including cortical pyramidal neurons, horn neurons in the spinal cord and Purkinje cells in cerebellum (at protein level).
Post Translational Modifications Isoform 1: Proteolytically cleaved by CASP3. Isoform 2: Phosphorylated at Ser-119 by PKA.
Function Hydrolyzes the second messenger 3',5'-cyclic AMP (cAMP), which is a key regulator of many important physiological processes. Isoform 1: Efficiently hydrolyzes cAMP. Isoform 2: Efficiently hydrolyzes cAMP. Isoform 3: Efficiently hydrolyzes cAMP. The phosphodiesterase activity is not affected by calcium, calmodulin or cyclic GMP (cGMP) levels. Does not hydrolyze cGMP. Isoform 4: Efficiently hydrolyzes cAMP. Isoform 6: Efficiently hydrolyzes cAMP. Isoform 7: Efficiently hydrolyzes cAMP.
Protein Name Camp-Specific 3' -5'-Cyclic Phosphodiesterase 4a
Dpde2
Pde46
Database Links Reactome: R-HSA-180024
Reactome: R-HSA-418555
Cellular Localisation Isoform 1: Cytoplasm
Perinuclear Region
Isoform 2: Cytoplasm
Cell Projection
Ruffle Membrane
Isoform 3: Cytoplasm
Cytosol
Isoform 4: Membrane
Peripheral Membrane Protein
Isoform 4 Has Propensity For Association With Membranes
Isoform 6: Cytoplasm
Isoform 7: Cytoplasm
Membrane
Predominantly Cytosolic
Alternative Antibody Names Anti-Camp-Specific 3' -5'-Cyclic Phosphodiesterase 4a antibody
Anti-Dpde2 antibody
Anti-Pde46 antibody
Anti-PDE4A antibody
Anti-DPDE2 antibody

Information sourced from Uniprot.org

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