• Immunofluorescence analysis of L929 cells using PDE11A Rabbit polyclonal antibody (STJ118561) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using PDE11A Rabbit polyclonal antibody (STJ118561) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PDE11A antibody (712-933) (STJ118561)

SKU:
STJ118561

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PDE11A (712-933) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PDE11A
Gene ID: 50940
Uniprot ID: PDE11_HUMAN
Immunogen Region: 712-933
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 712-933 of human PDE11A (NP_058649.3).
Immunogen Sequence: NNAFQAKSGSALAQLYGTSA TLEHHHFNHAVMILQSEGHN IFANLSSKEYSDLMQLLKQS ILATDLTLYFERRTEFFELV SKGEYDWNIKNHRDIFRSML MTACDLGAVTKPWEISRQVA ELVTSEFFEQGDRERLELKL TPSAIFDRNRKDELPRLQLE WIDSICMPLYQALVKVNVKL KPMLDSVATNRSKWEELHQK RLLASTASSSPASVMVAKED RN
Tissue Specificity Isoform 1 is present in prostate, pituitary, heart and liver. It is however not present in testis nor in penis, suggesting that weak inhibition by Tadalafil (Cialis) is not relevant (at protein level). Isoform 2 may be expressed in testis. Isoform 4 is expressed in adrenal cortex.
Function Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides cAMP and cGMP. Catalyzes the hydrolysis of both cAMP and cGMP to 5'-AMP and 5'-GMP, respectively.
Protein Name Dual 3' -5'-Cyclic-Amp And -Gmp Phosphodiesterase 11a
Camp And Cgmp Phosphodiesterase 11a
Database Links Reactome: R-HSA-418457
Reactome: R-HSA-418555
Cellular Localisation Cytoplasm
Cytosol
Alternative Antibody Names Anti-Dual 3' -5'-Cyclic-Amp And -Gmp Phosphodiesterase 11a antibody
Anti-Camp And Cgmp Phosphodiesterase 11a antibody
Anti-PDE11A antibody

Information sourced from Uniprot.org

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