| Host: |
Rabbit |
| Applications: |
WB/IP |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-PDCD6 (1-191) is suitable for use in Western Blot and Immunoprecipitation research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IP 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PDCD6 |
| Gene ID: |
10016 |
| Uniprot ID: |
PDCD6_HUMAN |
| Immunogen Region: |
1-191 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-191 of human PDCD6 (NP_037364.1). |
| Immunogen Sequence: |
MAAYSYRPGPGAGPGPAAGA ALPDQSFLWNVFQRVDKDRS GVISDTELQQALSNGTWTPF NPVTVRSIISMFDRENKAGV NFSEFTGVWKYITDWQNVFR TYDRDNSGMIDKNELKQALS GFGYRLSDQFHDILIRKFDR QGRGQIAFDDFIQGCIVLQR LTDIFRRYDTDQDGWIQVSY EQYLSMVFSIV |
| Function | Calcium sensor that plays a key role in processes such as endoplasmic reticulum (ER)-Golgi vesicular transport, endosomal biogenesis or membrane repair. Acts as an adapter that bridges unrelated proteins or stabilizes weak protein-protein complexes in response to calcium: calcium-binding triggers exposure of apolar surface, promoting interaction with different sets of proteins thanks to 3 different hydrophobic pockets, leading to translocation to membranes. Involved in ER-Golgi transport by promoting the association between PDCD6IP and TSG101, thereby bridging together the ESCRT-III and ESCRT-I complexes. Together with PEF1, acts as calcium-dependent adapter for the BCR(KLHL12) complex, a complex involved in ER-Golgi transport by regulating the size of COPII coats. In response to cytosolic calcium increase, the heterodimer formed with PEF1 interacts with, and bridges together the BCR(KLHL12) complex and SEC31 (SEC31A or SEC31B), promoting monoubiquitination of SEC31 and subsequent collagen export, which is required for neural crest specification. Involved in the regulation of the distribution and function of MCOLN1 in the endosomal pathway. Promotes localization and polymerization of TFG at endoplasmic reticulum exit site. Required for T-cell receptor-, Fas-, and glucocorticoid-induced apoptosis. May mediate Ca(2+)-regulated signals along the death pathway: interaction with DAPK1 can accelerate apoptotic cell death by increasing caspase-3 activity. Its role in apoptosis may however be indirect, as suggested by knockout experiments. May inhibit KDR/VEGFR2-dependent angiogenesis.the function involves inhibition of VEGF-induced phosphorylation of the Akt signaling pathway. In case of infection by HIV-1 virus, indirectly inhibits HIV-1 production by affecting viral Gag expression and distribution. Isoform 2: Has a lower Ca(2+) affinity than isoform 1. |
| Protein Name | Programmed Cell Death Protein 6Apoptosis-Linked Gene 2 Protein HomologAlg-2 |
| Cellular Localisation | Endoplasmic Reticulum MembranePeripheral Membrane ProteinCytoplasmic VesicleCopii-Coated Vesicle MembraneCytoplasmNucleusEndosomeInteraction With Rbm22 Induces Relocalization From The Cytoplasm To The NucleusTranslocated From The Cytoplasm To The Nucleus After Heat Shock Cell TreatmentAccumulates In Cytoplasmic Vesicle-Like Organelles After Heat Shock TreatmentWhich May Represent Stress GranulesIn Response To Calcium IncreaseRelocates From Cytoplasm To Copii Vesicle CoatLocalizes To Endoplasmic Reticulum Exit Site (Eres) |
| Alternative Antibody Names | Anti-Programmed Cell Death Protein 6 antibodyAnti-Apoptosis-Linked Gene 2 Protein Homolog antibodyAnti-Alg-2 antibodyAnti-PDCD6 antibodyAnti-ALG2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and PDCD6 knockout (KO) 293T cells, using [KO Validated] PDCD6 Rabbit polyclonal antibody (STJ11100800) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89736/358213/STJ11100800_1__35533.1713122537.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and PDCD6 knockout (KO) 293T cells, using [KO Validated] PDCD6 Rabbit polyclonal antibody (STJ11100800) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
