• STJ72822 (0. 1µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-PDCD4 antibody (210-221) (STJ72822)

SKU:
STJ72822-100

£303.50
Shipping:
Free Shipping
Current Stock:
Host: Goat
Applications: Pep-ELISA/WB/IHC
Reactivity: Human/Mouse/Rat/Dog/Pig/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-PDCD4 (210-221) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.1-0.3µg/ml
IHC-5µg/ml
ELISA-antibody detection limit dilution 1:128000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: PDCD4
Gene ID: 27250
Uniprot ID: PDCD4_HUMAN
Immunogen Region: 210-221
Accession Number: NP_055271.2; NP_663314.1; NP_001186421.1
Specificity: This antibody is expected to recognize all reported isoforms (NP_055271.2; NP_663314.1; NP_001186421.1).
Immunogen Sequence: EGKASHREMTSK
Post Translational Modifications Polyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation. Phosphorylated at Ser-67 by RPS6KB1 in response to mitogens.phosphorylation promotes proteasomal degradation of PDCD4.
Function Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA.
Protein Name Programmed Cell Death Protein 4
Neoplastic Transformation Inhibitor Protein
Nuclear Antigen H731-Like
Protein 197/15a
Database Links Reactome: R-HSA-8950505
Cellular Localisation Nucleus
Cytoplasm
Shuttles Between The Nucleus And Cytoplasm
Predominantly Nuclear Under Normal Growth Conditions
And When Phosphorylated At Ser-457
Alternative Antibody Names Anti-Programmed Cell Death Protein 4 antibody
Anti-Neoplastic Transformation Inhibitor Protein antibody
Anti-Nuclear Antigen H731-Like antibody
Anti-Protein 197/15a antibody
Anti-PDCD4 antibody
Anti-H731 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance