• Immunofluorescence analysis of NIH/3T3 cells using PDAP1 Rabbit polyclonal antibody (STJ11105692) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from Rat testis using PDAP1 Rabbit polyclonal antibody (STJ11105692) at 1:500 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime:60s.
  • Western blot analysis of various lysates using PDAP1 Rabbit polyclonal antibody (STJ11105692) at 1:500 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 30s.

Anti-PDAP1 antibody (1-100 aa) (STJ11105692)

SKU:
STJ11105692

Current Stock:
Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PDAP1
Gene ID: 11333
Uniprot ID: HAP28_HUMAN
Immunogen Region: 1-100 aa
Specificity: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PDAP1 (NP_055706.1).
Immunogen Sequence: MPKGGRKGGHKGRARQYTSP EEIDAQLQAEKQKAREEEEQ KEGGDGAAGDPKKEKKSLDS DESEDEEDDYQQKRKGVEGL IDIENPNRVAQTTKKVTQLD
Function Enhances PDGFA-stimulated cell growth in fibroblasts, but inhibits the mitogenic effect of PDGFB.
Protein Name 28 Kda Heat- And Acid-Stable Phosphoprotein
Pdgf-Associated Protein
Pap
Pdgfa-Associated Protein 1
Pap1
Database Links Reactome: R-HSA-6798695
Alternative Antibody Names Anti-28 Kda Heat- And Acid-Stable Phosphoprotein antibody
Anti-Pdgf-Associated Protein antibody
Anti-Pap antibody
Anti-Pdgfa-Associated Protein 1 antibody
Anti-Pap1 antibody
Anti-PDAP1 antibody
Anti-HASPP28 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance