• Western blot analysis of extracts of K-562 cells, using PCYT1A Rabbit monoclonal antibody (STJ11103592) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PCYT1A antibody (268-367) [S2MR] (STJ11103592)

SKU:
STJ11103592

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-PCYT1A (268-367) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PCYT1A
Gene ID: 5130
Uniprot ID: PCY1A_HUMAN
Immunogen Region: 268-367
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 268-367 of human PCYT1A (P49585).
Immunogen Sequence: EEKSIDLIQKWEEKSREFIG SFLEMFGPEGALKHMLKEGK GRMLQAISPKQSPSSSPTRE RSPSPSFRWPFSGKTSPPCS PANLSRHKAAAYDISEDEED
Tissue Specificity Brain, placenta, liver, fetal and adult lung.
Post Translational Modifications The serine residues of the C-terminus are phosphorylated. The inactive soluble form is stabilized by phosphorylation, the active membrane bound form is promoted by anionic lipids or diacylglycerol, and is stabilized by dephosphorylation. Monoubiquitinated by the SCF(FBXL2) complex, leading to proteasomal degradation.
Function Catalyzes the key rate-limiting step in the CDP-choline pathway for phosphatidylcholine biosynthesis.
Protein Name Choline-Phosphate Cytidylyltransferase A
Cct-Alpha
Ctp -Phosphocholine Cytidylyltransferase A
Cct A
Ct A
Phosphorylcholine Transferase A
Database Links Reactome: R-HSA-1483191
Cellular Localisation Cytoplasm
Cytosol
Membrane
Peripheral Membrane Protein
Endoplasmic Reticulum
Nucleus
It Can Interconvert Between An Inactive Cytosolic Form And An Active Membrane-Bound Form
Alternative Antibody Names Anti-Choline-Phosphate Cytidylyltransferase A antibody
Anti-Cct-Alpha antibody
Anti-Ctp -Phosphocholine Cytidylyltransferase A antibody
Anti-Cct A antibody
Anti-Ct A antibody
Anti-Phosphorylcholine Transferase A antibody
Anti-PCYT1A antibody
Anti-CTPCT antibody
Anti-PCYT1 antibody

Information sourced from Uniprot.org

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