• Immunohistochemistry of baboon lung with Anti-PCSK9 primary antibody (STJ501873) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-PCSK9 antibody (30-60) (STJ501873)

SKU:
STJ501873-100

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Host: Rabbit
Applications: ELISA/IP/WB
Reactivity: Human/Monkey/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PCSK9 (30-60) is suitable for use in ELISA, Immunoprecipitation and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Purification: Affinity Purified
Concentration: 0.85-1.25 µg/µl
Dilution Range: WB: 1:500
ELISA: 1:10, 000
IP: 1:200
IHC: 1:50-1:150
Storage Instruction: Store at-20°C for long term storage. Avoid freeze-thaw cycles.
Gene Symbol: Pcsk9
Gene ID: 100102
Uniprot ID: PCSK9_MOUSE
Immunogen Region: 30-60
Immunogen: Synthetic peptide taken within amino acid region 30-60 on the NARC1 protein.
Tissue Specificity Hepatocytes, kidney mesenchymal cells, intestinal ileum, colon epithelia and embryonic brain telencephalon neurons.
Post Translational Modifications Cleavage by furin and PCSK5 generates a truncated inactive protein that is unable to induce LDLR degradation. Undergoes autocatalytic cleavage in the endoplasmic reticulum to release the propeptide from the N-terminus and the cleavage of the propeptide is strictly required for its maturation and activation. The cleaved propeptide however remains associated with the catalytic domain through non-covalent interactions, preventing potential substrates from accessing its active site. As a result, it is secreted from cells as a propeptide-containing, enzymatically inactive protein. Phosphorylation protects the propeptide against proteolysis.
Function Crucial player in the regulation of plasma cholesterol homeostasis. Binds to low-density lipid receptor family members: low density lipoprotein receptor (LDLR), very low density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER) and apolipoprotein receptor 2 (LRP8/APOER2), and promotes their degradation in intracellular acidic compartments. Acts via a non-proteolytic mechanism to enhance the degradation of the hepatic LDLR through a clathrin LDLRAP1/ARH-mediated pathway. May prevent the recycling of LDLR from endosomes to the cell surface or direct it to lysosomes for degradation. Can induce ubiquitination of LDLR leading to its subsequent degradation. Inhibits intracellular degradation of APOB via the autophagosome/lysosome pathway in a LDLR-independent manner. Involved in the disposal of non-acetylated intermediates of BACE1 in the early secretory pathway. Inhibits epithelial Na(+) channel (ENaC)-mediated Na(+) absorption by reducing ENaC surface expression primarily by increasing its proteasomal degradation. Regulates neuronal apoptosis via modulation of LRP8/APOER2 levels and related anti-apoptotic signaling pathways.
Protein Name Proprotein Convertase Subtilisin/Kexin Type 9
Neural Apoptosis-Regulated Convertase 1
Narc-1
Proprotein Convertase 9
Pc9
Subtilisin/Kexin-Like Protease Pc9
Database Links Reactome: R-MMU-381426
Reactome: -MMU-8866427
Reactome: -MMU-8957275
Reactome: -MMU-8964038
Cellular Localisation Cytoplasm
Secreted
Endosome
Lysosome
Cell Surface
Endoplasmic Reticulum
Golgi Apparatus
Autocatalytic Cleavage Is Required To Transport It From The Endoplasmic Reticulum To The Golgi Apparatus And For The Secretion Of The Mature Protein
Localizes To The Endoplasmic Reticulum In The Absence Of Ldlr And Co-Localizes To The Cell Surface And To The Endosomes/Lysosomes In The Presence Of Ldlr
The Sorting To The Cell Surface And Endosomes Is Required In Order To Fully Promote Ldlr Degradation
Alternative Antibody Names Anti-Proprotein Convertase Subtilisin/Kexin Type 9 antibody
Anti-Neural Apoptosis-Regulated Convertase 1 antibody
Anti-Narc-1 antibody
Anti-Proprotein Convertase 9 antibody
Anti-Pc9 antibody
Anti-Subtilisin/Kexin-Like Protease Pc9 antibody
Anti-Pcsk9 antibody
Anti-Narc1 antibody

Information sourced from Uniprot.org

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