• Anti-PCNA antibody (162-261) [S3MR] (STJ11101413)
  • Immunohistochemistry analysis of PCNA in paraffin-embedded human esophagus tissue using PCNA Rabbit monoclonal antibody (STJ11101413) at a dilution of 1:8000 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of PCNA in paraffin-embedded rat testis tissue using PCNA Rabbit monoclonal antibody (STJ11101413) at a dilution of 1:8000 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of PCNA in paraffin-embedded human esophageal cancer tissue using PCNA Rabbit monoclonal antibody (STJ11101413) at a dilution of 1:8000 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of PCNA in paraffin-embedded human colon carcinoma tissue using PCNA Rabbit monoclonal antibody (STJ11101413) at a dilution of 1:8000 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of PCNA in paraffin-embedded mouse testis tissue using PCNA Rabbit monoclonal antibody (STJ11101413) at a dilution of 1:8000 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of PCNA in paraffin-embedded human colon tissue using PCNA Rabbit monoclonal antibody (STJ11101413) at a dilution of 1:8000 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of PCNA in paraffin-embedded rat colon tissue using PCNA Rabbit monoclonal antibody (STJ11101413) at a dilution of 1:8000 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates, using PCNA Rabbit monoclonal antibody (STJ11101413) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PCNA antibody (162-261) [S3MR] (STJ11101413)

SKU:
STJ11101413

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat/African green monkey
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-PCNA (162-261) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:2000-1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PCNA
Gene ID: 5111
Uniprot ID: PCNA_HUMAN
Immunogen Region: 162-261
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 162-261 of human PCNA (NP_002583.1).
Immunogen Sequence: CAKDGVKFSASGELGNGNIK LSQTSNVDKEEEAVTIEMNE PVQLTFALRYLNFFTKATPL SSTVTLSMSADVPLVVEYKI ADMGHLKYYLAPKIEDEEGS
Post Translational Modifications Phosphorylated. Phosphorylation at Tyr-211 by EGFR stabilizes chromatin-associated PCNA. Acetylated by CREBBP and p300/EP300.preferentially acetylated by CREBBP on Lys-80, Lys-13 and Lys-14 and on Lys-77 by p300/EP300 upon loading on chromatin in response to UV irradiation. Lysine acetylation disrupts association with chromatin, hence promoting PCNA ubiquitination and proteasomal degradation in response to UV damage in a CREBBP- and EP300-dependent manner. Acetylation disrupts interaction with NUDT15 and promotes degradation. Ubiquitinated. Following DNA damage, can be either monoubiquitinated to stimulate direct bypass of DNA lesions by specialized DNA polymerases or polyubiquitinated to promote recombination-dependent DNA synthesis across DNA lesions by template switching mechanisms. Following induction of replication stress, monoubiquitinated by the UBE2B-RAD18 complex on Lys-164, leading to recruit translesion (TLS) polymerases, which are able to synthesize across DNA lesions in a potentially error-prone manner. An error-free pathway also exists and requires non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH. This error-free pathway, also known as template switching, employs recombination mechanisms to synthesize across the lesion, using as a template the undamaged, newly synthesized strand of the sister chromatid. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis. Sumoylated during S phase. Methylated on glutamate residues by ARMT1/C6orf211.
Function Auxiliary protein of DNA polymerase delta and epsilon, is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.
Protein Name Proliferating Cell Nuclear Antigen
Pcna
Cyclin
Database Links Reactome: R-HSA-110312
Reactome: R-HSA-110314
Reactome: R-HSA-110320
Reactome: R-HSA-1362277
Reactome: R-HSA-174411
Reactome: R-HSA-174414
Reactome: R-HSA-174417
Reactome: R-HSA-174437
Reactome: R-HSA-4615885
Reactome: R-HSA-5358565
Reactome: R-HSA-5358606
Reactome: R-HSA-5651801
Reactome: R-HSA-5655862
Reactome: R-HSA-5656121
Reactome: R-HSA-5656169
Reactome: R-HSA-5685942
Reactome: R-HSA-5696397
Reactome: R-HSA-5696400
Reactome: R-HSA-6782135
Reactome: R-HSA-6782210
Reactome: R-HSA-6804114
Reactome: R-HSA-69091
Reactome: R-HSA-69166
Reactome: R-HSA-69183
Reactome: R-HSA-69205
Reactome: R-HSA-8866654
Cellular Localisation Nucleus
Colocalizes With Crebbp
Ep300 And Pold1 To Sites Of Dna Damage
Forms Nuclear Foci Representing Sites Of Ongoing Dna Replication And Vary In Morphology And Number During S Phase
Co-Localizes With Smarca5/Snf2h And Baz1b/Wstf At Replication Foci During S Phase
Together With Apex2
Is Redistributed In Discrete Nuclear Foci In Presence Of Oxidative Dna Damaging Agents
Alternative Antibody Names Anti-Proliferating Cell Nuclear Antigen antibody
Anti-Pcna antibody
Anti-Cyclin antibody
Anti-PCNA antibody

Information sourced from Uniprot.org

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