Anti-PCMT1 antibody (62-286) (STJ28767)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal PCMT1 (62-286) antibody for use in WB, IF, ICC, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:100IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PCMT1 |
| Gene ID | 5110 |
| Uniprot ID | PIMT_HUMAN |
| Immunogen Region | 62-286 |
| Immunogen Sequence | KSGGASHSELIHNLRKNGII KTDKVFEVMLATDRSHYAKC NPYMDSPQSIGFQATISAPH MHAYALELLFDQLHEGAKAL DVGSGSGILTACFARMVGCT GKVIGIDHIKELVDDSVNNV RKDDPTLLSSGRVQLVVGDG RMGYAEEAPYDAIHVGAAAP VVPQALIDQLKPGGRLILPV GPAGGNQMLEQYDKLQDGSI KMKPLMGVIYVPLTDKEKQW SRDEL |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 62-286 of human PCMT1 (NP_001238978.1). |
Additional Info
| Function | Initiates the repair of damaged proteins by catalyzing methyl esterification of L-isoaspartyl and D-aspartyl residues produced by spontaneous isomerization and racemization of L-aspartyl and L-asparaginyl residues in aging peptides and proteins. Acts on EIF4EBP2, microtubule-associated protein 2, calreticulin, clathrin light chains a and b, Ubiquitin C-terminal hydrolase isozyme L1, phosphatidylethanolamine-binding protein 1, stathmin, beta-synuclein and alpha-synuclein. |
| Protein Name | Protein-L-Isoaspartate(D-Aspartate O-MethyltransferasePimtL-Isoaspartyl Protein Carboxyl MethyltransferaseProtein L-Isoaspartyl/D-Aspartyl MethyltransferaseProtein-Beta-Aspartate Methyltransferase |
| Database Links | Reactome: R-HSA-5676934 |
| Cellular Localisation | CytoplasmCytosol |
| Alternative Antibody Names | Anti-Protein-L-Isoaspartate(D-Aspartate O-Methyltransferase antibodyAnti-Pimt antibodyAnti-L-Isoaspartyl Protein Carboxyl Methyltransferase antibodyAnti-Protein L-Isoaspartyl/D-Aspartyl Methyltransferase antibodyAnti-Protein-Beta-Aspartate Methyltransferase antibodyAnti-PCMT1 antibody |
Information sourced from Uniprot.org