• Immunoprecipitation analysis of 200 Mu g extracts of HepG2 cells using 1 Mu g PCMT1 antibody (STJ28767). Western blot was performed from the immunoprecipitate using PCMT1 antibody (STJ28767) at a dilution of 1:1000.
  • Immunofluorescence analysis of HeLa cells using PCMT1 Rabbit polyclonal antibody (STJ28767). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using PCMT1 Rabbit polyclonal antibody (STJ28767). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PCMT1 antibody (62-286) (STJ28767)

SKU:
STJ28767

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PCMT1 (62-286) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PCMT1
Gene ID: 5110
Uniprot ID: PIMT_HUMAN
Immunogen Region: 62-286
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 62-286 of human PCMT1 (NP_001238978.1).
Immunogen Sequence: KSGGASHSELIHNLRKNGII KTDKVFEVMLATDRSHYAKC NPYMDSPQSIGFQATISAPH MHAYALELLFDQLHEGAKAL DVGSGSGILTACFARMVGCT GKVIGIDHIKELVDDSVNNV RKDDPTLLSSGRVQLVVGDG RMGYAEEAPYDAIHVGAAAP VVPQALIDQLKPGGRLILPV GPAGGNQMLEQYDKLQDGSI KMKPLMGVIYVPLTDKEKQW SRDEL
Function Initiates the repair of damaged proteins by catalyzing methyl esterification of L-isoaspartyl and D-aspartyl residues produced by spontaneous isomerization and racemization of L-aspartyl and L-asparaginyl residues in aging peptides and proteins. Acts on EIF4EBP2, microtubule-associated protein 2, calreticulin, clathrin light chains a and b, Ubiquitin C-terminal hydrolase isozyme L1, phosphatidylethanolamine-binding protein 1, stathmin, beta-synuclein and alpha-synuclein.
Protein Name Protein-L-Isoaspartate(D-Aspartate O-Methyltransferase
Pimt
L-Isoaspartyl Protein Carboxyl Methyltransferase
Protein L-Isoaspartyl/D-Aspartyl Methyltransferase
Protein-Beta-Aspartate Methyltransferase
Database Links Reactome: R-HSA-5676934
Cellular Localisation Cytoplasm
Cytosol
Alternative Antibody Names Anti-Protein-L-Isoaspartate(D-Aspartate O-Methyltransferase antibody
Anti-Pimt antibody
Anti-L-Isoaspartyl Protein Carboxyl Methyltransferase antibody
Anti-Protein L-Isoaspartyl/D-Aspartyl Methyltransferase antibody
Anti-Protein-Beta-Aspartate Methyltransferase antibody
Anti-PCMT1 antibody

Information sourced from Uniprot.org

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