Anti-PCM1 antibody (N-Term) (STJ13101647)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide from n-terminal region of human PCM1 conjugated to blue carrier protein was used as the antigen.
General Information
| Short Description | Rabbit polyclonal anti-PCM1 (N-Term) for use in IHC and WB in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG purified |
| Dilution Range | IHC, WB. A dilution of 1:1000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20Β°C for long term storage and refrigerated at 2-8Β°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | PCM1 |
| Gene ID | 5108 |
| Uniprot ID | PCM1_HUMAN |
| Immunogen | A synthetic peptide from n-terminal region of human PCM1 conjugated to blue carrier protein was used as the antigen. |
| Immunogen Region | N-Term |
| Specificity | Specific for PCM1. |
Additional Info
| Post Translational Modifications | Ubiquitinated. Undergoes monoubiquitination catalyzed by the E3 ubiquitin-protein ligase MIB1 in proliferating cells, preventing cilia formation. Monoubiquitination by MIB1 is inhibited in response to cellular stress, such as ultraviolet light (UV) radiation or heat shock, resulting in cilia formation initiation. Variant Ser-159 is phosphorylated. Phosphorylated on multiple serine and threonine residues by DYRK3 during the G2-to-M transition, after the nuclear-envelope breakdown. Phosphorylation by DYRK3 promotes disassembly of pericentriolar material. Phosphorylation at Ser-372 mediated by PLK4 is required to maintain the integrity of centriolar satellites. |
| Function | Required for centrosome assembly and function. Essential for the correct localization of several centrosomal proteins including CEP250, CETN3, PCNT and NEK2. Required to anchor microtubules to the centrosome. Also involved in cilium biogenesis by recruiting the BBSome, a ciliary protein complex involved in cilium biogenesis, to the centriolar satellites. Recruits the tubulin polyglutamylase complex (TPGC) to centriolar satellites. |
| Protein Name | Pericentriolar Material 1 ProteinPcm-1Hpcm-1 |
| Database Links | Reactome: R-HSA-2565942Reactome: R-HSA-380259Reactome: R-HSA-380270Reactome: R-HSA-380284Reactome: R-HSA-380320Reactome: R-HSA-5620912Reactome: R-HSA-8854518 |
| Cellular Localisation | CytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeCytoplasmic GranuleCentriolar SatelliteCilium Basal BodyRecruitment To The Centrosome Requires Microtubules And DyneinThe Majority Of The Protein Dissociates From The Centrosome During Metaphase And Subsequently Localizes To The Cleavage Site In TelophaseDisplaced From Centriolar Satellites And Centrosome In Response To Cellular StressSuch As Ultraviolet Light (Uv) Radiation Or Heat ShockIn A Process That Requires P38 Map Kinase Signaling |
| Alternative Antibody Names | Anti-Pericentriolar Material 1 Protein antibodyAnti-Pcm-1 antibodyAnti-Hpcm-1 antibodyAnti-PCM1 antibody |
Information sourced from Uniprot.org