Anti-PCK2/PEPCK-M antibody (Internal) (STJ70781)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-PCK2/PEPCK-M (Internal) is suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry research applications. |
| Applications | Pep-ELISA/WB/IF/FC |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Dog/Pig/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx 70kDa band observed in lysates of cell lines A431, HEK293 HepG2 and NIH3T3 (calculated MW of 70.7kDa according to Human NP_004554.3 and 70.5kDa according to Mouse NP_083270.2). R |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | PCK2 |
| Gene ID | 5106 |
| Uniprot ID | PCKGM_HUMAN |
| Accession Number | NP_004554.3; NP_001278485.1 |
| Immunogen Region | Internal |
| Immunogen Sequence | KPWKPGDKEPCAH |
| Specificity | No cross-reactivity with PCK1. |
Additional Info
| Function | Mitochondrial phosphoenolpyruvate carboxykinase that catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. Can play an active role in glyceroneogenesis and gluconeogenesis. |
| Protein Name | Phosphoenolpyruvate Carboxykinase Gtp - MitochondrialPepck-MPhosphoenolpyruvate Carboxykinase 2 - MitochondrialMtpck2 |
| Database Links | Reactome: R-HSA-70263 |
| Cellular Localisation | Mitochondrion |
| Alternative Antibody Names | Anti-Phosphoenolpyruvate Carboxykinase Gtp - Mitochondrial antibodyAnti-Pepck-M antibodyAnti-Phosphoenolpyruvate Carboxykinase 2 - Mitochondrial antibodyAnti-Mtpck2 antibodyAnti-PCK2 antibodyAnti-PEPCK2 antibody |
Information sourced from Uniprot.org