• Western blot analysis of extracts of various cell lines, using PCK1 antibody (STJ24916) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of extracts of HepG2 cells, using PCK1 antibody (STJ24916) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Immunofluorescence analysis of rat kidney using PCK1 rabbit polyclonal antibody (STJ24916) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of human kidney cancer using PCK1 rabbit polyclonal antibody (STJ24916) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse kidney using PCK1 rabbit polyclonal antibody (STJ24916) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HepG2 cells using PCK1 rabbit polyclonal antibody (STJ24916) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 300 Mu g extracts of mouse liver cells using 3 Mu g PCK1 antibody (STJ24916). Western blot was performed from the immunoprecipitate using PCK1 antibody (STJ24916) at a dilution of 1:1000.

Anti-PCK1 antibody (490-622) (STJ24916)

SKU:
STJ24916

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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PCK1 (490-622) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PCK1
Gene ID: 5105
Uniprot ID: PCKGC_HUMAN
Immunogen Region: 490-622
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 490-622 of human PCK1 (NP_002582.3).
Immunogen Sequence: FGKYLAHWLSMAQHPAAKLP KIFHVNWFRKDKEGKFLWPG FGENSRVLEWMFNRIDGKAS TKLTPIGYIPKEDALNLKGL GHINMMELFSISKEFWEKEV EDIEKYLEDQVNADLPCEIE REILALKQRISQM
Tissue Specificity Major sites of expression are liver, kidney and adipocytes.
Post Translational Modifications Acetylated. Lysine acetylation by p300/EP300 is increased on high glucose conditions. Lysine acetylation promotes ubiquitination by UBR5. Acetylation is enhanced in the presence of BAG6. Deacetylated by SIRT2. Deacetylation of Lys-91 is carried out by SIRT1 and depends on PCK1 phosphorylation levels. Phosphorylated in a GSK3B-mediated pathway.phosphorylation affects the efficiency of SIRT1-mediated deacetylation, and regulates PCK1 ubiquitination and degradation. Phosphorylation at Ser-90 by AKT1 reduces the binding affinity to oxaloacetate and promotes the protein kinase activity: phosphorylated PCK1 translocates to the endoplasmic reticulum, where it phosphorylates INSIG1 and INSIG2. Ubiquitination by UBR5 leads to proteasomal degradation.
Function Cytosolic phosphoenolpyruvate carboxykinase that catalyzes the reversible decarboxylation and phosphorylation of oxaloacetate (OAA) and acts as the rate-limiting enzyme in gluconeogenesis. Regulates cataplerosis and anaplerosis, the processes that control the levels of metabolic intermediates in the citric acid cycle. At low glucose levels, it catalyzes the cataplerotic conversion of oxaloacetate to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. At high glucose levels, it catalyzes the anaplerotic conversion of phosphoenolpyruvate to oxaloacetate. Acts as a regulator of formation and maintenance of memory CD8(+) T-cells: up-regulated in these cells, where it generates phosphoenolpyruvate, via gluconeogenesis. The resultant phosphoenolpyruvate flows to glycogen and pentose phosphate pathway, which is essential for memory CD8(+) T-cells homeostasis. In addition to the phosphoenolpyruvate carboxykinase activity, also acts as a protein kinase when phosphorylated at Ser-90: phosphorylation at Ser-90 by AKT1 reduces the binding affinity to oxaloacetate and promotes an atypical serine protein kinase activity using GTP as donor. The protein kinase activity regulates lipogenesis: upon phosphorylation at Ser-90, translocates to the endoplasmic reticulum and catalyzes phosphorylation of INSIG proteins (INSIG1 and INSIG2), thereby disrupting the interaction between INSIG proteins and SCAP and promoting nuclear translocation of SREBP proteins (SREBF1/SREBP1 or SREBF2/SREBP2) and subsequent transcription of downstream lipogenesis-related genes.
Protein Name Phosphoenolpyruvate Carboxykinase - Cytosolic Gtp
Pepck-C
Serine-Protein Kinase Pck1
Database Links Reactome: R-HSA-2161541
Reactome: R-HSA-381340
Reactome: R-HSA-70263
Reactome: R-HSA-9615017
Reactome: R-HSA-9632974
Cellular Localisation Cytoplasm
Cytosol
Endoplasmic Reticulum
Phosphorylation At Ser-90 Promotes Translocation To The Endoplasmic Reticulum
Alternative Antibody Names Anti-Phosphoenolpyruvate Carboxykinase - Cytosolic Gtp antibody
Anti-Pepck-C antibody
Anti-Serine-Protein Kinase Pck1 antibody
Anti-PCK1 antibody
Anti-PEPCK1 antibody

Information sourced from Uniprot.org

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