• Immunofluorescence analysis of L929 cells using PCDHB4 Rabbit polyclonal antibody (STJ119774) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using PCDHB4 Rabbit polyclonal antibody (STJ119774) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-PCDHB4 antibody (28-300) (STJ119774)

SKU:
STJ119774

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PCDHB4 (28-300) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PCDHB4
Gene ID: 56131
Uniprot ID: PCDB4_HUMAN
Immunogen Region: 28-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 28-300 of human PCDHB4 (NP_061761.1).
Immunogen Sequence: EPIRYSVLEETESGSFVAHL AKDLGLGIGELASRSARVLS DDDKQRLQLDRQTGDLLLRE KLDREELCGPIEPCVLHFQV FLEMPVQFFQGELLIQDIND HSPIFPEREVLLKILENSQP GTLFPLLIAEDLDVGSNGLQ KYTISPNSHFHILTRNHSEG KKYPDLVQDKPLDREEQPEF SLTLVALDGGSPPRSGTVMV RILIMDINDNAPEFVHTPYG VQVLENSPLDSPIVRVLAR
Function Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain.
Protein Name Protocadherin Beta-4
Pcdh-Beta-4
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Protocadherin Beta-4 antibody
Anti-Pcdh-Beta-4 antibody
Anti-PCDHB4 antibody

Information sourced from Uniprot.org

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