• Immunohistochemistry analysis of paraffin-embedded rat brain using hnRNP E1/PCBP1 Rabbit polyclonal antibody (STJ11104507) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse testis using hnRNP E1/PCBP1 Rabbit polyclonal antibody (STJ11104507) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using hnRNP E1/PCBP1 Rabbit polyclonal antibody (STJ11104507) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts from wild type (WT) and hnRNP E1/PCBP1 knockout (KO) HeLa cells, using hnRNP E1/PCBP1 antibody (STJ11104507) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of various cell lines, using hnRNP E1/PCBP1 antibody (STJ11104507) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PCBP1 antibody (100-200) (STJ11104507)

SKU:
STJ11104507

£129.50 - £639.50
Current Stock:
Host: rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Poly (Rc-Binding Protein 1 (100-200) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PCBP1
Gene ID: 5093
Uniprot ID: PCBP1_HUMAN
Immunogen Region: 100-200
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human hnRNP E1/PCBP1 (NP_006187.2).
Immunogen Sequence: LRLVVPATQCGSLIGKGGCK IKEIRESTGAQVQVAGDMLP NSTERAITIAGVPQSVTECV KQICLVMLETLSQSPQGRVM TIPYQPMPASSPVICAGGQD R
Tissue Specificity Abundantly expressed in skeletal muscle, thymus and peripheral blood leukocytes while a lower expression is observed in prostate, spleen, testis, ovary, small intestine, heart, liver, adrenal and thyroid glands.
Post Translational Modifications Phosphorylated.lowers poly(rC)-binding activity.
Function Single-stranded nucleic acid binding protein that binds preferentially to oligo dC. Together with PCBP2, required for erythropoiesis, possibly by regulating mRNA splicing. (Microbial infection) In case of infection by poliovirus, plays a role in initiation of viral RNA replication in concert with the viral protein 3CD.
Protein Name Poly(Rc-Binding Protein 1
Alpha-Cp1
Heterogeneous Nuclear Ribonucleoprotein E1
Hnrnp E1
Nucleic Acid-Binding Protein Sub2.3
Database Links Reactome: R-HSA-72163
Reactome: R-HSA-72203
Cellular Localisation Nucleus
Cytoplasm
Loosely Bound In The Nucleus
May Shuttle Between The Nucleus And The Cytoplasm
Alternative Antibody Names Anti-Poly(Rc-Binding Protein 1 antibody
Anti-Alpha-Cp1 antibody
Anti-Heterogeneous Nuclear Ribonucleoprotein E1 antibody
Anti-Hnrnp E1 antibody
Anti-Nucleic Acid-Binding Protein Sub2.3 antibody
Anti-PCBP1 antibody

Information sourced from Uniprot.org

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