• Western blot analysis of extracts of various cell lines, using Pyruvate carboxylase (PC) rabbit monoclonal antibody (STJ11102403) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of various cell lines, using Pyruvate carboxylase (PC) Rabbit monoclonal antibody (STJ11102403) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PC antibody (1-100) [S3MR] (STJ11102403)

SKU:
STJ11102403

£212.50 - £501.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-PCB (1-100) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PC
Gene ID: 5091
Uniprot ID: PYC_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Pyruvate carboxylase (PC) (P11498).
Immunogen Sequence: MLKFRTVHGGLRLLGIRRTS TAPAASPNVRRLEYKPIKKV MVANRGEIAIRVFRACTELG IRTVAIYSEQDTGQMHRQKA DEAYLIGRGLAPVQAYLHIP
Post Translational Modifications Acetylation of Lys-748 might play a role in catalytic activity regulation.
Function Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate.
Protein Name Pyruvate Carboxylase - Mitochondrial
Pyruvic Carboxylase
Pcb
Database Links Reactome: R-HSA-196780
Reactome: R-HSA-3371599
Reactome: R-HSA-70263
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Pyruvate Carboxylase - Mitochondrial antibody
Anti-Pyruvic Carboxylase antibody
Anti-Pcb antibody
Anti-PC antibody

Information sourced from Uniprot.org

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