• Western blot analysis of extracts of various cell lines, using PBXIP1 antibody (STJ111417) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of extracts of various cell lines, using PBXIP1 antibody (STJ111417) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PBXIP1 antibody (1-190) (STJ111417)

SKU:
STJ111417

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PBXIP1 (1-190) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PBXIP1
Gene ID: 57326
Uniprot ID: PBIP1_HUMAN
Immunogen Region: 1-190
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human PBXIP1 (NP_065385.2).
Immunogen Sequence: MASCPDSDNSWVLAGSESLP VETLGPASRMDPESERALQA PHSPSKTDGKELAGTMDGEG TLFQTESPQSGSILTEETEV KGTLEGDVCGVEPPGPGDTV VQGDLQETTVVTGLGPDTQD LEGQSPPQSLPSTPKAAWIR EEGRCSSSDDDTDVDMEGLR RRRGREAGPPQPMVPLAVEN QAGGEGAGGE
Tissue Specificity Expressed in early hematopoietic precursors.
Function Regulator of pre-B-cell leukemia transcription factors (BPXs) function. Inhibits the binding of PBX1-HOX complex to DNA and blocks the transcriptional activity of E2A-PBX1. Tethers estrogen receptor-alpha (ESR1) to microtubules and allows them to influence estrogen receptors-alpha signaling.
Protein Name Pre-B-Cell Leukemia Transcription Factor-Interacting Protein 1
Hematopoietic Pbx-Interacting Protein
Cellular Localisation Cytoplasm
Cytoskeleton
Nucleus
Shuttles Between The Nucleus And The Cytosol
Mainly Localized In The Cytoplasm
Associated With Microtubules
Detected In Small Amounts In The Nucleus
Alternative Antibody Names Anti-Pre-B-Cell Leukemia Transcription Factor-Interacting Protein 1 antibody
Anti-Hematopoietic Pbx-Interacting Protein antibody
Anti-PBXIP1 antibody
Anti-HPIP antibody

Information sourced from Uniprot.org

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