• Western blot analysis of extracts of various cell lines, using PBRM1 rabbit monoclonal antibody (STJ11101234) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
  • Immunohistochemistry analysis of paraffin-embedded rat ovary using PBRM1 rabbit monoclonal antibody (STJ11101234) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human appendix using PBRM1 rabbit monoclonal antibody (STJ11101234) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using PBRM1 rabbit monoclonal antibody (STJ11101234) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon using PBRM1 rabbit monoclonal antibody (STJ11101234) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human spleen using PBRM1 rabbit monoclonal antibody (STJ11101234) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using PBRM1 rabbit monoclonal antibody (STJ11101234) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-PBRM1 antibody (530-680) [S4MR] (STJ11101234)

SKU:
STJ11101234

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-PBRM1 (530-680) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PBRM1
Gene ID: 55193
Uniprot ID: PB1_HUMAN
Immunogen Region: 530-680
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 530-680 of human PBRM1 (Q86U86).
Immunogen Sequence: VLEAREPGSGRRLCDLFMVK PSKKDYPDYYKIILEPMDLK IIEHNIRNDKYAGEEGMIED MKLMFRNARHYNEEGSQVYN DAHILEKLLKEKRKELGPLP DDDDMASPKLKLSRKSGISP KKSKYMTPMQQKLNEVYEAV KNYTDKRGRRL
Tissue Specificity Widely expressed.
Function Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Required for the stability of the SWI/SNF chromatin remodeling complex SWI/SNF-B (PBAF). Acts as a negative regulator of cell proliferation.
Protein Name Protein Polybromo-1
Hpb1
Brg1-Associated Factor 180
Baf180
Polybromo-1d
Database Links Reactome: R-HSA-3214858
Reactome: R-HSA-8939243
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Protein Polybromo-1 antibody
Anti-Hpb1 antibody
Anti-Brg1-Associated Factor 180 antibody
Anti-Baf180 antibody
Anti-Polybromo-1d antibody
Anti-PBRM1 antibody
Anti-BAF180 antibody
Anti-PB1 antibody

Information sourced from Uniprot.org

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