| Short Description : | Rabbit polyclonal PBRM1 (1-230) antibody for use in WB, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IP/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PBRM1 |
| Gene ID: | 55193 |
| Uniprot ID: | PB1_HUMAN |
| Immunogen Region: | 1-230 |
| Immunogen Sequence: | MGSKRRRATSPSSSVSGDFD DGHHSVSTPGPSRKRRRLSN LPTVDPIAVCHELYNTIRDY KDEQGRLLCELFIRAPKRRN QPDYYEVVSQPIDLMKIQQK LKMEEYDDVNLLTADFQLLF NNAKSYYKPDSPEYKAACKL WDLYLRTRNEFVQKGEADDE DDDEDGQDNQGTVTEGSSPA YLKEILEQLLEAIVVATNPS GRLISELFQKLPSKVQYPDY YAIIKEPIDL |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human PBRM1 (NP_060783.3). |
| Tissue Specificity | Widely expressed. |
| Function | Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Required for the stability of the SWI/SNF chromatin remodeling complex SWI/SNF-B (PBAF). Acts as a negative regulator of cell proliferation. |
| Protein Name | Protein Polybromo-1Hpb1Brg1-Associated Factor 180Baf180Polybromo-1d |
| Database Links | Reactome: R-HSA-3214858Reactome: R-HSA-8939243 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Protein Polybromo-1 antibodyAnti-Hpb1 antibodyAnti-Brg1-Associated Factor 180 antibodyAnti-Baf180 antibodyAnti-Polybromo-1d antibodyAnti-PBRM1 antibodyAnti-BAF180 antibodyAnti-PB1 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and ATG4D knockout (KO) HeLa cells, using [KO Validated] ATG4D Rabbit polyclonal antibody (STJ11100070) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89140/357590/STJ11100070_1__34146.1713121727.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and ATG4D knockout (KO) HeLa cells, using [KO Validated] ATG4D Rabbit polyclonal antibody (STJ11100070) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
