• Western blot analysis of various lysates using PBK Rabbit polyclonal antibody (STJ11104418) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of lysates from HeLa cells using PBK Rabbit polyclonal antibody (STJ11104418) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime:1s.
  • Western blot analysis of lysates from wild type (WT) and PBK knockout (KO) 293T cells using PBK Rabbit polyclonal antibody (STJ11104418) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 1s.

Anti-PBK antibody (1-322) (STJ11104418)

SKU:
STJ11104418

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Lymphokine-Activated Killer T-Cell-Originated Protein Kinase (1-322) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PBK
Gene ID: 55872
Uniprot ID: TOPK_HUMAN
Immunogen Region: 1-322
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-322 of human PBK (NP_060962.2).
Immunogen Sequence: MEGISNFKTPSKLSEKKKSV LCSTPTINIPASPFMQKLGF GTGVNVYLMKRSPRGLSHSP WAVKKINPICNDHYRSVYQK RLMDEAKILKSLHHPNIVGY RAFTEANDGSLCLAMEYGGE KSLNDLIEERYKASQDPFPA AIILKVALNMARGLKYLHQE KKLLHGDIKSSNVVIKGDFE TIKICDVGVSLPLDENMTVT DPEACYIGTEPWKPKEAVEE NGVITDKADIFAFGLTLWE
Tissue Specificity Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules.
Post Translational Modifications Phosphorylated.in a cell-cycle dependent manner at mitosis.
Function Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.
Protein Name Lymphokine-Activated Killer T-Cell-Originated Protein Kinase
Cancer/Testis Antigen 84
Ct84
Mapkk-Like Protein Kinase
Nori-3
Pdz-Binding Kinase
Spermatogenesis-Related Protein Kinase
Spk
T-Lak Cell-Originated Protein Kinase
Alternative Antibody Names Anti-Lymphokine-Activated Killer T-Cell-Originated Protein Kinase antibody
Anti-Cancer/Testis Antigen 84 antibody
Anti-Ct84 antibody
Anti-Mapkk-Like Protein Kinase antibody
Anti-Nori-3 antibody
Anti-Pdz-Binding Kinase antibody
Anti-Spermatogenesis-Related Protein Kinase antibody
Anti-Spk antibody
Anti-T-Lak Cell-Originated Protein Kinase antibody
Anti-PBK antibody
Anti-TOPK antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance