• Immunofluorescence analysis of HeLa cells using PAR4 Rabbit monoclonal antibody (STJ11102211) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using PAR4 Rabbit monoclonal antibody (STJ11102211) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using PAR4 Rabbit monoclonal antibody (STJ11102211) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PAWR antibody (241-340) [S1MR] (STJ11102211)

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STJ11102211

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-PAR4 (241-340) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PAWR
Gene ID: 5074
Uniprot ID: PAWR_HUMAN
Immunogen Region: 241-340
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 241-340 of human PAR4 (Q96IZ0).
Immunogen Sequence: YSRTDRSGFPRYNRDANVSG TLVSSSTLEKKIEDLEKEVV RERQENLRLVRLMQDKEEMI GKLKEEIDLLNRDLDDIEDE NEQLKQENKTLLKVVGQLTR
Tissue Specificity Widely expressed. Expression is elevated in various neurodegenerative diseases such as amyotrophic lateral sclerosis, Alzheimer, Parkinson and Huntington diseases and stroke. Down-regulated in several cancers.
Post Translational Modifications Preferentially phosphorylated at the Thr-163 by PKC in cancer cells.
Function Pro-apoptotic protein capable of selectively inducing apoptosis in cancer cells, sensitizing the cells to diverse apoptotic stimuli and causing regression of tumors in animal models. Induces apoptosis in certain cancer cells by activation of the Fas prodeath pathway and coparallel inhibition of NF-kappa-B transcriptional activity. Inhibits the transcriptional activation and augments the transcriptional repression mediated by WT1. Down-regulates the anti-apoptotic protein BCL2 via its interaction with WT1. Seems also to be a transcriptional repressor by itself. May be directly involved in regulating the amyloid precursor protein (APP) cleavage activity of BACE1.
Protein Name Prkc Apoptosis Wt1 Regulator Protein
Prostate Apoptosis Response 4 Protein
Par-4
Cellular Localisation Cytoplasm
Nucleus
Mainly Cytoplasmic In Absence Of Apoptosis Signal And In Normal Cells
Nuclear In Most Cancer Cell Lines
Nuclear Entry Seems To Be Essential But Not Sufficient For Apoptosis
Nuclear Localization Includes Nucleoplasm And Pml Nuclear Bodies
Alternative Antibody Names Anti-Prkc Apoptosis Wt1 Regulator Protein antibody
Anti-Prostate Apoptosis Response 4 Protein antibody
Anti-Par-4 antibody
Anti-PAWR antibody
Anti-PAR4 antibody

Information sourced from Uniprot.org

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