Anti-PASK antibody (1194-1323) (STJ116338)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal PASK (1194-1323) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PASK |
| Gene ID | 23178 |
| Uniprot ID | PASK_HUMAN |
| Immunogen Region | 1194-1323 |
| Immunogen Sequence | YTLVFEENPFCELEETVEAA IHPPYLVSKELMSLVSGLLQ PVPERRTTLEKLVTDPWVTQ PVNLADYTWEEVFRVNKPES GVLSAASLEMGNRSLSDVAQ AQELCGGPVPGEAPNGQGCL HPGDPRLLTS |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1194-1323 of human PASK (NP_055963.2). |
Additional Info
| Tissue Specificity | Ubiquitously expressed, with slightly higher expression in brain, prostate and testis. Reduced expression was found in placenta. Present in germ cells of testis and in the midpiece of sperm tails (at protein level). |
| Post Translational Modifications | Autophosphorylated on Thr-1161 and Thr-1165. Autophosphorylation is activated by phospholipids. |
| Function | Serine/threonine-protein kinase involved in energy homeostasis and protein translation. Phosphorylates EEF1A1, GYS1, PDX1 and RPS6. Probably plays a role under changing environmental conditions (oxygen, glucose, nutrition), rather than under standard conditions. Acts as a sensor involved in energy homeostasis: regulates glycogen synthase synthesis by mediating phosphorylation of GYS1, leading to GYS1 inactivation. May be involved in glucose-stimulated insulin production in pancreas and regulation of glucagon secretion by glucose in alpha cells.however such data require additional evidences. May play a role in regulation of protein translation by phosphorylating EEF1A1, leading to increase translation efficiency. May also participate in respiratory regulation. |
| Protein Name | Pas Domain-Containing Serine/Threonine-Protein KinasePas-KinasePaskinHpask |
| Database Links | |
| Cellular Localisation | CytoplasmNucleusLocalizes In The Nucleus Of Testis Germ Cells And In The Midpiece Of Sperm Tails |
| Alternative Antibody Names | Anti-Pas Domain-Containing Serine/Threonine-Protein Kinase antibodyAnti-Pas-Kinase antibodyAnti-Paskin antibodyAnti-Hpask antibodyAnti-PASK antibodyAnti-KIAA0135 antibody |
Information sourced from Uniprot.org