Anti-PARP3 antibody (Internal) (STJ71774)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-PARP3 (Internal) is suitable for use in ELISA and Immunohistochemistry research applications. |
| Applications | Pep-ELISA/IHC |
| Host | Goat |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:2000.WB: Preliminary experiments in lysates of cell lines Daudi, HeLa and HepG2 gave no specific signal but low background (at antibody concentration up to 1ยตg/ml).IHC: Paraffin embedded Hu |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20ยฐC on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | PARP3 |
| Gene ID | 10039 |
| Uniprot ID | PARP3_HUMAN |
| Accession Number | NP_001003931.2; NP_001003935.2 |
| Immunogen Region | Internal |
| Immunogen Sequence | YEDYNCTLNQTNIEN |
| Specificity | This antibody is expected to recognize both reported isoforms (NP_001003931.2; NP_001003935.2). |
Additional Info
| Post Translational Modifications | Auto-mono-ADP-ribosylated. |
| Function | Mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins and plays a key role in the response to DNA damage. Mediates mono-ADP-ribosylation of glutamate, aspartate or lysine residues on target proteins. In contrast to PARP1 and PARP2, it is not able to mediate poly-ADP-ribosylation. Involved in DNA repair by mediating mono-ADP-ribosylation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism, such as histone H2B, XRCC5 and XRCC6. ADP-ribosylation follows DNA damage and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Involved in single-strand break repair by catalyzing mono-ADP-ribosylation of histone H2B on 'Glu-2' (H2BE2ADPr) of nucleosomes containing nicked DNA. Cooperates with the XRCC5-XRCC6 (Ku80-Ku70) heterodimer to limit end-resection thereby promoting accurate NHEJ. Suppresses G-quadruplex (G4) structures in response to DNA damage. Associates with a number of DNA repair factors and is involved in the response to exogenous and endogenous DNA strand breaks. Together with APLF, promotes the retention of the LIG4-XRCC4 complex on chromatin and accelerate DNA ligation during non-homologous end-joining (NHEJ). May link the DNA damage surveillance network to the mitotic fidelity checkpoint. Acts as a negative regulator of immunoglobulin class switch recombination, probably by controlling the level of AICDA /AID on the chromatin. In addition to proteins, also able to ADP-ribosylate DNA: mediates DNA mono-ADP-ribosylation of DNA strand break termini via covalent addition of a single ADP-ribose moiety to a 5'- or 3'-terminal phosphate residues in DNA containing multiple strand breaks. |
| Protein Name | Protein Mono-Adp-Ribosyltransferase Parp3Adp-Ribosyltransferase Diphtheria Toxin-Like 3Artd3Dna Adp-Ribosyltransferase Parp3Irt1Nad(+ Adp-Ribosyltransferase 3Adprt-3Poly Adp-Ribose Polymerase 3Parp-3Hparp-3PolyAdp-Ribose Synthase 3Padprt-3 |
| Cellular Localisation | NucleusChromosomeCytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeCentrioleAlmost Exclusively Localized In The Nucleus And Appears In Numerous Small Foci And A Small Number Of Larger Foci Whereas A Centrosomal Location Has Not Been DetectedIn Response To Dna DamageLocalizes To Sites Of Double-Strand BreakAlso Localizes To Single-Strand BreaksPreferentially Localized To The Daughter Centriole |
| Alternative Antibody Names | Anti-Protein Mono-Adp-Ribosyltransferase Parp3 antibodyAnti-Adp-Ribosyltransferase Diphtheria Toxin-Like 3 antibodyAnti-Artd3 antibodyAnti-Dna Adp-Ribosyltransferase Parp3 antibodyAnti-Irt1 antibodyAnti-Nad(+ Adp-Ribosyltransferase 3 antibodyAnti-Adprt-3 antibodyAnti-Poly Adp-Ribose Polymerase 3 antibodyAnti-Parp-3 antibodyAnti-Hparp-3 antibodyAnti-PolyAdp-Ribose Synthase 3 antibodyAnti-Padprt-3 antibodyAnti-PARP3 antibodyAnti-ADPRT3 antibodyAnti-ADPRTL3 antibody |
Information sourced from Uniprot.org