Anti-PARP3 antibody (10-59 aa) (STJ94960)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human PARP3 at the amino acid range 10-59
General Information
| Short Description | Rabbit polyclonal anti-Protein mono-ADP-ribosyltransferase PARP3 ADP-ribosyltransferase 3 (10-59 aa) for use in IHC, IF and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | IHC 1:100-1:300ELISA 1:5000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PARP3 |
| Gene ID | 10039 |
| Uniprot ID | PARP3_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human PARP3 at the amino acid range 10-59 |
| Immunogen Region | 10-59 aa |
| Specificity | PARP-3 Polyclonal Antibody detects endogenous levels of PARP-3 protein. |
Additional Info
| Post Translational Modifications | Auto-mono-ADP-ribosylated. |
| Function | Mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins and plays a key role in the response to DNA damage. Mediates mono-ADP-ribosylation of glutamate, aspartate or lysine residues on target proteins. In contrast to PARP1 and PARP2, it is not able to mediate poly-ADP-ribosylation. Involved in DNA repair by mediating mono-ADP-ribosylation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism, such as histone H2B, XRCC5 and XRCC6. ADP-ribosylation follows DNA damage and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Involved in single-strand break repair by catalyzing mono-ADP-ribosylation of histone H2B on 'Glu-2' (H2BE2ADPr) of nucleosomes containing nicked DNA. Cooperates with the XRCC5-XRCC6 (Ku80-Ku70) heterodimer to limit end-resection thereby promoting accurate NHEJ. Suppresses G-quadruplex (G4) structures in response to DNA damage. Associates with a number of DNA repair factors and is involved in the response to exogenous and endogenous DNA strand breaks. Together with APLF, promotes the retention of the LIG4-XRCC4 complex on chromatin and accelerate DNA ligation during non-homologous end-joining (NHEJ). May link the DNA damage surveillance network to the mitotic fidelity checkpoint. Acts as a negative regulator of immunoglobulin class switch recombination, probably by controlling the level of AICDA /AID on the chromatin. In addition to proteins, also able to ADP-ribosylate DNA: mediates DNA mono-ADP-ribosylation of DNA strand break termini via covalent addition of a single ADP-ribose moiety to a 5'- or 3'-terminal phosphate residues in DNA containing multiple strand breaks. |
| Protein Name | Protein Mono-Adp-Ribosyltransferase Parp3Adp-Ribosyltransferase Diphtheria Toxin-Like 3Artd3Dna Adp-Ribosyltransferase Parp3Irt1Nad(+ Adp-Ribosyltransferase 3Adprt-3Poly Adp-Ribose Polymerase 3Parp-3Hparp-3PolyAdp-Ribose Synthase 3Padprt-3 |
| Database Links | |
| Cellular Localisation | NucleusChromosomeCytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeCentrioleAlmost Exclusively Localized In The Nucleus And Appears In Numerous Small Foci And A Small Number Of Larger Foci Whereas A Centrosomal Location Has Not Been DetectedIn Response To Dna DamageLocalizes To Sites Of Double-Strand BreakAlso Localizes To Single-Strand BreaksPreferentially Localized To The Daughter Centriole |
| Alternative Antibody Names | Anti-Protein Mono-Adp-Ribosyltransferase Parp3 antibodyAnti-Adp-Ribosyltransferase Diphtheria Toxin-Like 3 antibodyAnti-Artd3 antibodyAnti-Dna Adp-Ribosyltransferase Parp3 antibodyAnti-Irt1 antibodyAnti-Nad(+ Adp-Ribosyltransferase 3 antibodyAnti-Adprt-3 antibodyAnti-Poly Adp-Ribose Polymerase 3 antibodyAnti-Parp-3 antibodyAnti-Hparp-3 antibodyAnti-PolyAdp-Ribose Synthase 3 antibodyAnti-Padprt-3 antibodyAnti-PARP3 antibodyAnti-ADPRT3 antibodyAnti-ADPRTL3 antibody |
Information sourced from Uniprot.org