• STJ70331 (0. 001µg/ml) staining of HeLa (A) and Jurkat (B) lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70331 (0. 001µg/ml) staining of Human Cerebellum (A) , Human Frontal Cortex (B) , Human Hippocampus (C) Mouse Fetal Brain (D) , Mouse Brain (E) and Rat Brain (F) lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • Immunofluorescence staining of MCF7 (A) and Hela (B) cells with 5ug/ml STJ70331 antibody. Detected with Rabbit anti-goat IgG-Alexafluor488 antibody at 1:1000. Nuclei Counterstained with DAPI.

Anti-PARK7/DJ-1 antibody (C-Term) (STJ70331)

SKU:
STJ70331-100

Shipping:
Free Shipping
Current Stock:
Host: Goat
Applications: Pep-ELISA/WB/IF/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-PARK7/DJ-1 (C-Term) is suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IF-Strong expression of the protein seen around the nucleus. Dividing cells appear to show enhanced expression. 5µg/ml
ELISA-antibody detection limit dilution 1:128000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: PARK7
Gene ID: 11315
Uniprot ID: PARK7_HUMAN
Immunogen Region: C-Term
Accession Number: NP_009193.2
Specificity: Reported variants (NP_001116849.1; NP_009193.2) represent the same protein.
Immunogen Sequence: AAQVKAPLVLKD
Post Translational Modifications Sumoylated on Lys-130 by PIAS2 or PIAS4.which is enhanced after ultraviolet irradiation and essential for cell-growth promoting activity and transforming activity. Cys-106 is easily oxidized to sulfinic acid. Undergoes cleavage of a C-terminal peptide and subsequent activation of protease activity in response to oxidative stress.
Function Multifunctional protein with controversial molecular function which plays an important role in cell protection against oxidative stress and cell death acting as oxidative stress sensor and redox-sensitive chaperone and protease. It is involved in neuroprotective mechanisms like the stabilization of NFE2L2 and PINK1 proteins, male fertility as a positive regulator of androgen signaling pathway as well as cell growth and transformation through, for instance, the modulation of NF-kappa-B signaling pathway. Has been described as a protein and nucleotide deglycase that catalyzes the deglycation of the Maillard adducts formed between amino groups of proteins or nucleotides and reactive carbonyl groups of glyoxals. But this function is rebuted by other works. As a protein deglycase, repairs methylglyoxal- and glyoxal-glycated proteins, and releases repaired proteins and lactate or glycolate, respectively. Deglycates cysteine, arginine and lysine residues in proteins, and thus reactivates these proteins by reversing glycation by glyoxals. Acts on early glycation intermediates (hemithioacetals and aminocarbinols), preventing the formation of advanced glycation endproducts (AGE) that cause irreversible damage. Also functions as a nucleotide deglycase able to repair glycated guanine in the free nucleotide pool (GTP, GDP, GMP, dGTP) and in DNA and RNA. Is thus involved in a major nucleotide repair system named guanine glycation repair (GG repair), dedicated to reversing methylglyoxal and glyoxal damage via nucleotide sanitization and direct nucleic acid repair. Protects histones from adduction by methylglyoxal, controls the levels of methylglyoxal-derived argininine modifications on chromatin. Able to remove the glycations and restore histone 3, histone glycation disrupts both local and global chromatin architecture by altering histone-DNA interactions as well as histone acetylation and ubiquitination levels. Displays a very low glyoxalase activity that may reflect its deglycase activity. Eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death. Required for correct mitochondrial morphology and function as well as for autophagy of dysfunctional mitochondria. Plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking. Regulates astrocyte inflammatory responses, may modulate lipid rafts-dependent endocytosis in astrocytes and neuronal cells. In pancreatic islets, involved in the maintenance of mitochondrial reactive oxygen species (ROS) levels and glucose homeostasis in an age- and diet dependent manner. Protects pancreatic beta cells from cell death induced by inflammatory and cytotoxic setting. Binds to a number of mRNAs containing multiple copies of GG or CC motifs and partially inhibits their translation but dissociates following oxidative stress. Metal-binding protein able to bind copper as well as toxic mercury ions, enhances the cell protection mechanism against induced metal toxicity. In macrophages, interacts with the NADPH oxidase subunit NCF1 to direct NADPH oxidase-dependent ROS production, and protects against sepsis.
Protein Name Parkinson Disease Protein 7
Maillard Deglycase
Oncogene Dj1
Parkinsonism-Associated Deglycase
Protein Dj-1
Dj-1
Protein/Nucleic Acid Deglycase Dj-1
Database Links Reactome: R-HSA-3899300
Reactome: R-HSA-9613829
Reactome: R-HSA-9615710
Reactome: R-HSA-9646399
Cellular Localisation Cell Membrane
Lipid-Anchor
Cytoplasm
Nucleus
Membrane Raft
Mitochondrion
Endoplasmic Reticulum
Under Normal Conditions
Located Predominantly In The Cytoplasm And
To A Lesser Extent
In The Nucleus And Mitochondrion
Translocates To The Mitochondrion And Subsequently To The Nucleus In Response To Oxidative Stress And Exerts An Increased Cytoprotective Effect Against Oxidative Damage
Detected In Tau Inclusions In Brains From Neurodegenerative Disease Patients
Membrane Raft Localization In Astrocytes And Neuronal Cells Requires Palmitoylation
Alternative Antibody Names Anti-Parkinson Disease Protein 7 antibody
Anti-Maillard Deglycase antibody
Anti-Oncogene Dj1 antibody
Anti-Parkinsonism-Associated Deglycase antibody
Anti-Protein Dj-1 antibody
Anti-Dj-1 antibody
Anti-Protein/Nucleic Acid Deglycase Dj-1 antibody
Anti-PARK7 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance