• Western blot analysis of various lysates using PARD6A Rabbit polyclonal antibody (STJ24893) at 1:200 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PARD6A antibody (1-100) (STJ24893)

SKU:
STJ24893

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PARD6A (1-100) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PARD6A
Gene ID: 50855
Uniprot ID: PAR6A_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PARD6A (Q9NPB6).
Immunogen Sequence: MARPQRTPARSPDSIVEVKS KFDAEFRRFALPRASVSGFQ EFSRLLRAVHQIPGLDVLLG YTDAHGDLLPLTNDDSLHRA LASGPPPLRLLVQKRAEADS
Tissue Specificity Expressed in pancreas, skeletal muscle, brain and heart. Weakly expressed in kidney and placenta.
Post Translational Modifications Phosphorylated by the TGF-beta receptor.
Function Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in the formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Regulates centrosome organization and function. Essential for the centrosomal recruitment of key proteins that control centrosomal microtubule organization.
Protein Name Partitioning Defective 6 Homolog Alpha
Par-6
Par-6 Alpha
Par-6a
Par6c
Tax Interaction Protein 40
Tip-40
Database Links Reactome: R-HSA-2173791
Reactome: R-HSA-420029
Reactome: R-HSA-4608870
Reactome: R-HSA-9013148
Reactome: R-HSA-9013149
Reactome: R-HSA-9013420
Reactome: R-HSA-9013424
Cellular Localisation Cytoplasm
Cell Membrane
Cell Projection
Ruffle
Cell Junction
Tight Junction
Cytoskeleton
Microtubule Organizing Center
Centrosome
Centriolar Satellite
Colocalizes With Gtp-Bound Cdc42 Or Rac1 At Membrane Ruffles And With Pard3 And Prkci At Epithelial Tight Junctions
Recruited To The Centrosome By A Microtubule And Dynein-Dynactin-Dependent Mechanism
Alternative Antibody Names Anti-Partitioning Defective 6 Homolog Alpha antibody
Anti-Par-6 antibody
Anti-Par-6 Alpha antibody
Anti-Par-6a antibody
Anti-Par6c antibody
Anti-Tax Interaction Protein 40 antibody
Anti-Tip-40 antibody
Anti-PARD6A antibody
Anti-PAR6A antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance