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Anti-PAR6alpha/PARD6A antibody (C-Term) (STJ70485)

STJ70485 (2µg/ml) staining of Jurkat (A) and U251 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
STJ70485 (10µg/ml) staining of paraffin embedded Human Pancreas. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
STJ70485 Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-PAR6alpha/PARD6A antibody (C-Term) (STJ70485)

SKU:

STJ70485-100

£303.50

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General Information
Product Properties
Target Information
Additional Info
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Host:	Goat
Applications:	Pep-ELISA/WB/IHC/FC
Reactivity:	Human
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Goat polyclonal antibody anti-PAR6alpha/PARD6A (C-Term) is suitable for use in ELISA, Western Blot, Immunohistochemistry and Flow Cytometry research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification:	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration:	0.5 mg/mL
Dilution Range:	WB-1-3µg/ml IHC-10µg/ml FC-Flow cytometric analysis of Jurkat cells. 10ug/ml ELISA-antibody detection limit dilution 1:8000.
Storage Instruction:	Store at-20°C on receipt and minimise freeze-thaw cycles.

Gene Symbol:	PARD6A
Gene ID:	50855
Uniprot ID:	PAR6A_HUMAN
Immunogen Region:	C-Term
Accession Number:	NP_058644.1; NP_001032358.1
Specificity:	This antibody is expected to recognise both reported isoforms (NP_058644.1; NP_001032358.1).
Immunogen Sequence:	GSRIRGDGSGFSL

Post Translational Modifications	Phosphorylated by the TGF-beta receptor.
Function	Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in the formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Regulates centrosome organization and function. Essential for the centrosomal recruitment of key proteins that control centrosomal microtubule organization.
Protein Name	Partitioning Defective 6 Homolog Alpha Par-6 Par-6 Alpha Par-6a Par6c Tax Interaction Protein 40 Tip-40
Database Links	Reactome: R-HSA-2173791 Reactome: R-HSA-420029 Reactome: R-HSA-4608870 Reactome: R-HSA-9013148 Reactome: R-HSA-9013149 Reactome: R-HSA-9013420 Reactome: R-HSA-9013424
Cellular Localisation	Cytoplasm Cell Membrane Cell Projection Ruffle Cell Junction Tight Junction Cytoskeleton Microtubule Organizing Center Centrosome Centriolar Satellite Colocalizes With Gtp-Bound Cdc42 Or Rac1 At Membrane Ruffles And With Pard3 And Prkci At Epithelial Tight Junctions Recruited To The Centrosome By A Microtubule And Dynein-Dynactin-Dependent Mechanism
Alternative Antibody Names	Anti-Partitioning Defective 6 Homolog Alpha antibody Anti-Par-6 antibody Anti-Par-6 Alpha antibody Anti-Par-6a antibody Anti-Par6c antibody Anti-Tax Interaction Protein 40 antibody Anti-Tip-40 antibody Anti-PARD6A antibody Anti-PAR6A antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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