• Immunohistochemistry analysis of MARK3 in paraffin-embedded mouse kidney using MARK3 Rabbit polyclonal antibody (STJ11103026) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of MARK3 in paraffin-embedded human liver cancer using MARK3 Rabbit polyclonal antibody (STJ11103026) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of MARK3 in paraffin-embedded rat heart using MARK3 Rabbit polyclonal antibody (STJ11103026) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from SH-SY5Y cells using MARK3 Rabbit polyclonal antibody (STJ11103026) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime:90s.

Anti-MARK3 antibody (1-100) (STJ11103026)

SKU:
STJ11103026

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MARK3 (1-100) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MARK3
Gene ID: 4140
Uniprot ID: MARK3_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MARK3 (NP_001122390.2).
Immunogen Sequence: MSTRTPLPTVNERDTENHTS HGDGRQEVTSRTSRSGARCR NSIASCADEQPHIGNYRLLK TIGKGNFAKVKLARHILTGR EVAIKIIDKTQLNPTSLQKL
Tissue Specificity Ubiquitous.
Post Translational Modifications Phosphorylated at Thr-211 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Phosphorylation at Thr-564 by PRKCZ/aPKC inhibits the kinase activity.
Function Serine/threonine-protein kinase. Involved in the specific phosphorylation of microtubule-associated proteins for MAP2 and MAP4. Phosphorylates the microtubule-associated protein MAPT/TAU. Phosphorylates CDC25C on 'Ser-216'. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus. Regulates localization and activity of MITF by mediating its phosphorylation, promoting subsequent interaction between MITF and 14-3-3 and retention in the cytosol. Negatively regulates the Hippo signaling pathway and antagonizes the phosphorylation of LATS1. Cooperates with DLG5 to inhibit the kinase activity of STK3/MST2 toward LATS1. Phosphorylates PKP2 and KSR1.
Protein Name Map/Microtubule Affinity-Regulating Kinase 3
C-Tak1
Ctak1
Cdc25c-Associated Protein Kinase 1
Elkl Motif Kinase 2
Emk-2
Protein Kinase Stk10
Ser/Thr Protein Kinase Par-1
Par-1a
Serine/Threonine-Protein Kinase P78
Database Links Reactome: R-HSA-5673000
Reactome: R-HSA-5674135
Reactome: R-HSA-5675221
Reactome: R-HSA-6802946
Reactome: R-HSA-6802948
Reactome: R-HSA-6802952
Reactome: R-HSA-6802955
Reactome: R-HSA-9649948
Reactome: R-HSA-9656223
Cellular Localisation Cell Membrane
Peripheral Membrane Protein
Cell Projection
Dendrite
Cytoplasm
Alternative Antibody Names Anti-Map/Microtubule Affinity-Regulating Kinase 3 antibody
Anti-C-Tak1 antibody
Anti-Ctak1 antibody
Anti-Cdc25c-Associated Protein Kinase 1 antibody
Anti-Elkl Motif Kinase 2 antibody
Anti-Emk-2 antibody
Anti-Protein Kinase Stk10 antibody
Anti-Ser/Thr Protein Kinase Par-1 antibody
Anti-Par-1a antibody
Anti-Serine/Threonine-Protein Kinase P78 antibody
Anti-MARK3 antibody
Anti-CTAK1 antibody
Anti-EMK2 antibody

Information sourced from Uniprot.org

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