• STJ70483 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear, plasma membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70483 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing Golgi and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70483 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-PALS/MPP5 antibody (N-Term) (STJ70483)

SKU:
STJ70483-100

£303.50
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Host: Goat
Applications: Pep-ELISA/IF/IHC/FC
Reactivity: Human/Cow/Dog/Pig
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-PALS/MPP5 (N-Term) is suitable for use in ELISA, Immunofluorescence, Immunohistochemistry and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IHC-5µg/ml
IF-Strong expression of the protein seen in U2OS and HeLa cells. 10µg/ml
FC-Flow cytometric analysis of HeLa cells. 10ug/ml
ELISA-antibody detection limit dilution 1:32000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: PALS1
Gene ID: 64398
Uniprot ID: PALS1_HUMAN
Immunogen Region: N-Term
Accession Number: NP_071919.2; NP_001243479.1
Immunogen Sequence: NKASPPFPLISNAQ
Function Plays a role in tight junction biogenesis and in the establishment of cell polarity in epithelial cells. Also involved in adherens junction biogenesis by ensuring correct localization of the exocyst complex protein EXOC4/SEC8 which allows trafficking of adherens junction structural component CDH1 to the cell surface. Plays a role through its interaction with CDH5 in vascular lumen formation and endothelial membrane polarity. Required during embryonic and postnatal retinal development. Required for the maintenance of cerebellar progenitor cells in an undifferentiated proliferative state, preventing premature differentiation, and is required for cerebellar histogenesis, fissure formation, cerebellar layer organization and cortical development. Plays a role in neuronal progenitor cell survival, potentially via promotion of mTOR signaling. Plays a role in the radial and longitudinal extension of the myelin sheath in Schwann cells. May modulate SC6A1/GAT1-mediated GABA uptake by stabilizing the transporter. Plays a role in the T-cell receptor-mediated activation of NF-kappa-B. Required for localization of EZR to the apical membrane of parietal cells and may play a role in the dynamic remodeling of the apical cytoskeleton. Required for the normal polarized localization of the vesicular marker STX4. Required for the correct trafficking of the myelin proteins PMP22 and MAG. Involved in promoting phosphorylation and cytoplasmic retention of transcriptional coactivators YAP1 and WWTR1/TAZ which leads to suppression of TGFB1-dependent transcription of target genes such as CCN2/CTGF, SERPINE1/PAI1, SNAI1/SNAIL1 and SMAD7. (Microbial infection) Acts as an interaction partner for human coronaviruses SARS-CoV and, probably, SARS-CoV-2 envelope protein E which results in delayed formation of tight junctions and disregulation of cell polarity.
Protein Name Protein Pals1
Maguk P55 Subfamily Member 5
Membrane Protein - Palmitoylated 5
Protein Associated With Lin-7 1
Database Links Reactome: R-HSA-420029
Reactome: R-HSA-9692912
Reactome: R-HSA-9705677
Cellular Localisation Golgi Apparatus
Cell Membrane
Peripheral Membrane Protein
Endomembrane System
Cell Junction
Tight Junction
Adherens Junction
Cell Projection
Axon
Perikaryon
Apical Cell Membrane
Localized To The Tight Junctions Of Epithelial Cells
Localized To The Golgi Apparatus In T Lymphocytes
Localized To A Subset Of Intracellular Vesicles
Localized To The Purkinje Cell Body And Axon
Localized To Intercellular Junctions In Vascular Endothelial Cells
Localized To Schmidt-Lanterman Incisures
The Adaxonal Domain
And The Inner Part Of Paranodal Loops In Myelinating Schwann Cells Of The Sciatic Nerve
Localized To Apical Membrane Domains Of The Outer Limiting Membrane (Olm) Junctions In The Retina
Colocalizes With Crb1 At The Olm
Apical To The Adherens Junction
Colocalizes With Mpp1 In The Retina At The Olm
Colocalizes With Mpp3 To The Subapical Region Of Adherens Junctions In The Retina Olm
Endoplasmic Reticulum-Golgi Intermediate Compartment
(Microbial Infection) Following Infection By Human Sars Coronavirus
Partially Localized At The Site Of Viral Replication
The Endoplasmic Reticulum-Golgi Intermediate Compartment
Reducing Its Levels At Cell-Cell Contacts Which Results In Delayed Formation Of Tight Junctions And Affects Establishment Of Cell Polarity
Alternative Antibody Names Anti-Protein Pals1 antibody
Anti-Maguk P55 Subfamily Member 5 antibody
Anti-Membrane Protein - Palmitoylated 5 antibody
Anti-Protein Associated With Lin-7 1 antibody
Anti-PALS1 antibody
Anti-MPP5 antibody

Information sourced from Uniprot.org

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