• Immunohistochemistry of paraffin-embedded Mouse spleen using PADI4 antibody (STJ118628) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded Rat bone marrow using PADI4 antibody (STJ118628) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded Human tonsil using PADI4 antibody (STJ118628) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded Rat spleen using PADI4 antibody (STJ118628) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using PADI4 antibody (STJ118628) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.

Anti-PADI4 antibody (STJ118628)

SKU:
STJ118628

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Host: Rabbit
Applications: WB, IHC
Reactivity: Human, Mouse, Rat
Short Description: Rabbit polyclonal anti-PADI4 antibody is suitable for use in Western Blot and Immunohistochemistry.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:500-1:2000
IHC: 1:50-1:200
Uniprot ID: PADI4_HUMAN
Gene ID: 23569
Gene Symbol: PADI4
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 374-550 of human PADI4 (NP_036519.2).
Tissue Specificity Expressed in eosinophils and neutrophils, not expressed in peripheral monocytes or lymphocytes.
Post Translational Modifications Autocitrullination at Arg-372 and Arg-374 inactivates the enzyme.
Function Catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance. Citrullinates histone H1 at 'Arg-54' (to form H1R54ci), histone H3 at 'Arg-2', 'Arg-8', 'Arg-17' and/or 'Arg-26' (to form H3R2ci, H3R8ci, H3R17ci, H3R26ci, respectively) and histone H4 at 'Arg-3' (to form H4R3ci). Acts as a key regulator of stem cell maintenance by mediating citrullination of histone H1: citrullination of 'Arg-54' of histone H1 (H1R54ci) results in H1 displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance. Promotes profound chromatin decondensation during the innate immune response to infection in neutrophils by mediating formation of H1R54ci. Citrullination of histone H3 prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1.
Protein Name Protein-Arginine Deiminase Type-4
Hl-60 Pad
Peptidylarginine Deiminase Iv
Protein-Arginine Deiminase Type Iv
Database Links Reactome: R-HSA-3247509
Cellular Localisation Cytoplasm
Nucleus
Cytoplasmic Granule
Cytoplasmic Granules Of Eosinophils And Neutrophils
Alternative Antibody Names Anti-Protein-Arginine Deiminase Type-4 antibody
Anti-Hl-60 Pad antibody
Anti-Peptidylarginine Deiminase Iv antibody
Anti-Protein-Arginine Deiminase Type Iv antibody
Anti-PADI4 antibody
Anti-PAD4 antibody
Anti-PADI5 antibody
Anti-PDI5 antibody

Information sourced from Uniprot.org

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