Anti-PADI4 antibody (374-560) (STJ118628)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal PADI4 (374-560) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PADI4 |
| Gene ID | 23569 |
| Uniprot ID | PADI4_HUMAN |
| Immunogen Region | 374-560 |
| Immunogen Sequence | VFRVAPWIMTPNTQPPQEVY ACSIFENEDFLKSVTTLAMK AKCKLTICPEEENMDDQWMQ DEMEIGYIQAPHKTLPVVFD SPRNRGLKEFPIKRVMGPDF GYVTRGPQTGGISGLDSFGN LEVSPPVTVRGKEYPLGRIL FGDSCYPSNDSRQMHQALQD FLSAQQVQAPVKLYSDWLSV GHVDEFLSFVPAPDRKGFRL LLASPRSCYKLFQEQQNEGH GEALLFEGIKKKKQQKIKN |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 290-663 of human PADI4 (NP_036519.2). |
Additional Info
| Tissue Specificity | Expressed in eosinophils and neutrophils, not expressed in peripheral monocytes or lymphocytes. |
| Post Translational Modifications | Autocitrullination at Arg-372 and Arg-374 inactivates the enzyme. |
| Function | Catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance. Citrullinates histone H1 at 'Arg-54' (to form H1R54ci), histone H3 at 'Arg-2', 'Arg-8', 'Arg-17' and/or 'Arg-26' (to form H3R2ci, H3R8ci, H3R17ci, H3R26ci, respectively) and histone H4 at 'Arg-3' (to form H4R3ci). Acts as a key regulator of stem cell maintenance by mediating citrullination of histone H1: citrullination of 'Arg-54' of histone H1 (H1R54ci) results in H1 displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance. Promotes profound chromatin decondensation during the innate immune response to infection in neutrophils by mediating formation of H1R54ci. Required for the formation of neutrophil extracellular traps (NETs).NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation. Citrullination of histone H3 prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1. |
| Protein Name | Protein-Arginine Deiminase Type-4Hl-60 PadPeptidylarginine Deiminase IvProtein-Arginine Deiminase Type Iv |
| Database Links | Reactome: R-HSA-3247509 |
| Cellular Localisation | CytoplasmNucleusCytoplasmic GranuleCytoplasmic Granules Of Eosinophils And Neutrophils |
| Alternative Antibody Names | Anti-Protein-Arginine Deiminase Type-4 antibodyAnti-Hl-60 Pad antibodyAnti-Peptidylarginine Deiminase Iv antibodyAnti-Protein-Arginine Deiminase Type Iv antibodyAnti-PADI4 antibodyAnti-PAD4 antibodyAnti-PADI5 antibodyAnti-PDI5 antibody |
Information sourced from Uniprot.org