• Western blot analysis of extracts of various cell lines, using PACSIN3 antibody (STJ111362) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of extracts of various cell lines, using PACSIN3 antibody (STJ111362) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PACSIN3 antibody (175-424) (STJ111362)

SKU:
STJ111362

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PACSIN3 (175-424) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PACSIN3
Gene ID: 29763
Uniprot ID: PACN3_HUMAN
Immunogen Region: 175-424
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 175-424 of human PACSIN3 (NP_057307.2).
Immunogen Sequence: KADSAVSQEQLRKLQERVER CAKEAEKTKAQYEQTLAELH RYTPRYMEDMEQAFETCQAA ERQRLLFFKDMLLTLHQHLD LSSSEKFHELHRDLHQGIEA ASDEEDLRWWRSTHGPGMAM NWPQFEEWSLDTQRTISRKE KGGRSPDEVTLTSIVPTRDG TAPPPQSPGSPGTGQDEEWS DEESPRKAATGVRVRALYDY AGQEADELSFRAGEELLKMS EEDEQGWCQGQLQSGRIGL
Tissue Specificity Widely expressed, with highest levels in heart and skeletal muscle, intermediate levels in placenta, liver and pancreas, and very low levels in brain, lung and kidney.
Post Translational Modifications Phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC).
Function Plays a role in endocytosis and regulates internalization of plasma membrane proteins. Overexpression impairs internalization of SLC2A1/GLUT1 and TRPV4 and increases the levels of SLC2A1/GLUT1 and TRPV4 at the cell membrane. Inhibits the TRPV4 calcium channel activity.
Protein Name Protein Kinase C And Casein Kinase Substrate In Neurons Protein 3
Sh3 Domain-Containing Protein 6511
Database Links Reactome: R-HSA-8856828
Cellular Localisation Cytoplasm
Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Detected At The Inner Aspect Of The Plasma Membrane In Myotubes
Alternative Antibody Names Anti-Protein Kinase C And Casein Kinase Substrate In Neurons Protein 3 antibody
Anti-Sh3 Domain-Containing Protein 6511 antibody
Anti-PACSIN3 antibody

Information sourced from Uniprot.org

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