Anti-PACSIN1 antibody (N-Term) (STJ70099)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-PACSIN1 (N-Term) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Applications | Pep-ELISA/WB/IHC |
| Host | Goat |
| Reactivity | Human/Dog/Pig/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 50kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 51.1kDa according to NP_065855.1). Recommended concentration: 0.03-0.1µg/ml.An additional band of unkn |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | PACSIN1 |
| Gene ID | 29993 |
| Uniprot ID | PACN1_HUMAN |
| Accession Number | NP_065855.1 |
| Immunogen Region | N-Term |
| Immunogen Sequence | SSSYDEASLAPEET |
| Specificity | Reported variants represent identical protein (NP_065855.1; NP_001186512.1). |
Additional Info
| Post Translational Modifications | Phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC). |
| Function | Plays a role in the reorganization of the microtubule cytoskeleton via its interaction with MAPT.this decreases microtubule stability and inhibits MAPT-induced microtubule polymerization. Plays a role in cellular transport processes by recruiting DNM1, DNM2 and DNM3 to membranes. Plays a role in the reorganization of the actin cytoskeleton and in neuron morphogenesis via its interaction with COBL and WASL, and by recruiting COBL to the cell cortex. Plays a role in the regulation of neurite formation, neurite branching and the regulation of neurite length. Required for normal synaptic vesicle endocytosis.this process retrieves previously released neurotransmitters to accommodate multiple cycles of neurotransmission. Required for normal excitatory and inhibitory synaptic transmission. Binds to membranes via its F-BAR domain and mediates membrane tubulation. |
| Protein Name | Protein Kinase C And Casein Kinase Substrate In Neurons Protein 1Syndapin-1 |
| Database Links | Reactome: R-HSA-8856828 |
| Cellular Localisation | CytoplasmCell ProjectionSynapseSynaptosomeRuffle MembraneMembranePeripheral Membrane ProteinCytoplasmic Vesicle MembraneCytosolCell MembraneCytoplasmic SideColocalizes With Mapt In AxonsIn Primary Neuronal CulturesPresent At A High Level In Presynaptic Nerve Terminals And In The Cell BodyColocalizes With Dnm1 At Vesicular Structures In The Cell Body And NeuritesAssociates With Membranes Via Its F-Bar Domain |
| Alternative Antibody Names | Anti-Protein Kinase C And Casein Kinase Substrate In Neurons Protein 1 antibodyAnti-Syndapin-1 antibodyAnti-PACSIN1 antibodyAnti-KIAA1379 antibody |
Information sourced from Uniprot.org