• Western blot analysis of extracts of various cell lines, using EBP1/PA2G4 antibody (STJ115972) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST.
  • Western blot analysis of extracts from wild type (WT) and EBP1/PA2G4 knockout (KO) 293T (KO) cells, using EBP1/PA2G4 antibody (STJ115972) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PA2G4 antibody (1-394) (STJ115972)

SKU:
STJ115972

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PA2G4 (1-394) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PA2G4
Gene ID: 5036
Uniprot ID: PA2G4_HUMAN
Immunogen Region: 1-394
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-394 of human EBP1/PA2G4 (NP_006182.2).
Immunogen Sequence: MSGEDEQQEQTIAEDLVVTK YKMGGDIANRVLRSLVEASS SGVSVLSLCEKGDAMIMEET GKIFKKEKEMKKGIAFPTSI SVNNCVCHFSPLKSDQDYIL KEGDLVKIDLGVHVDGFIAN VAHTFVVDVAQGTQVTGRKA DVIKAAHLCAEAALRLVKPG NQNTQVTEAWNKVAHSFNCT PIEGMLSHQLKQHVIDGEKT IIQNPTDQQKKDHEKAEFEV HEVYAVDVLVSSGEGKAKD
Tissue Specificity Isoform 2 is undetectable whereas isoform 1 is strongly expressed in cancer cells (at protein level). Isoform 1 and isoform 2 are widely expressed, including heart, brain, lung, pancreas, skeletal muscle, kidney, placenta and liver.
Post Translational Modifications Phosphorylated on serine and threonine residues. Phosphorylation is enhanced by HRG treatment. Basal phosphorylation is PKC-dependent and HRG-induced phosphorylation is predominantly PKC-independent. Phosphorylation at Ser-361 by PKC/PRKCD regulates its nucleolar localization. In cancer cells, isoform 2 is polyubiquitinated leading to its proteasomal degradation and phosphorylation by PKC/PRKCD enhances polyubiquitination.
Function May play a role in a ERBB3-regulated signal transduction pathway. Seems be involved in growth regulation. Acts a corepressor of the androgen receptor (AR) and is regulated by the ERBB3 ligand neuregulin-1/heregulin (HRG). Inhibits transcription of some E2F1-regulated promoters, probably by recruiting histone acetylase (HAT) activity. Binds RNA. Associates with 28S, 18S and 5.8S mature rRNAs, several rRNA precursors and probably U3 small nucleolar RNA. May be involved in regulation of intermediate and late steps of rRNA processing. May be involved in ribosome assembly. Mediates cap-independent translation of specific viral IRESs (internal ribosomal entry site). Regulates cell proliferation, differentiation, and survival. Isoform 1 suppresses apoptosis whereas isoform 2 promotes cell differentiation.
Protein Name Proliferation-Associated Protein 2g4
Cell Cycle Protein P38-2g4 Homolog
Hg4-1
Erbb3-Binding Protein 1
Database Links Reactome: R-HSA-6798695
Cellular Localisation Isoform 1: Cytoplasm
Nucleus
Nucleolus
Translocates To The Nucleus Upon Treatment With Hrg
Phosphorylation At Ser-361 By Pkc/Prkcd Regulates Its Nucleolar Localization
Isoform 2: Cytoplasm
Alternative Antibody Names Anti-Proliferation-Associated Protein 2g4 antibody
Anti-Cell Cycle Protein P38-2g4 Homolog antibody
Anti-Hg4-1 antibody
Anti-Erbb3-Binding Protein 1 antibody
Anti-PA2G4 antibody
Anti-EBP1 antibody

Information sourced from Uniprot.org

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