Anti-p60 CAF1 antibody [R07-8Q6] (STJA0036961)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthesized peptide derived from human p60 CAF1
General Information
| Short Description | Rabbit monoclonal anti-p60 CAF1 for use in WB, IHC-P, ICC, IF, IP and FC in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ICC/IF/IP/FC |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R07-8Q6 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Chromatography |
| Dilution Range | WB 1:500-1:1000IHC 1:50-1:100IF 1:50-1:200IP 1:50FC 1:50-1:100 |
| Formulation | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | CHAF1B |
| Gene ID | 8208 |
| Uniprot ID | CAF1B_HUMAN |
| Immunogen | A synthesized peptide derived from human p60 CAF1 |
Additional Info
| Post Translational Modifications | Differentially phosphorylated during cell cycle. During mitosis the p60 subunit of inactive CAF-1 is hyperphosphorylated and displaced into the cytosol. Progressivly dephosphorylated from G1 to S and G2 phase. Phosphorylated p60 is recruited to chromatin undergoing DNA repair after UV irradiation in G1, S or G2 phases. |
| Function | Acts as a component of the histone chaperone complex chromatin assembly factor 1 (CAF-1), which assembles histone octamers onto DNA during replication and repair. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA.histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. |
| Protein Name | Chromatin Assembly Factor 1 Subunit BCaf-1 Subunit BChromatin Assembly Factor I P60 SubunitCaf-I 60 Kda SubunitCaf-I P60M-Phase Phosphoprotein 7 |
| Database Links | |
| Cellular Localisation | NucleusCytoplasmDna Replication FociCytoplasmic In M Phase |
| Alternative Antibody Names | Anti-Chromatin Assembly Factor 1 Subunit B antibodyAnti-Caf-1 Subunit B antibodyAnti-Chromatin Assembly Factor I P60 Subunit antibodyAnti-Caf-I 60 Kda Subunit antibodyAnti-Caf-I P60 antibodyAnti-M-Phase Phosphoprotein 7 antibodyAnti-CHAF1B antibodyAnti-CAF1A antibodyAnti-CAF1P60 antibodyAnti-MPHOSPH7 antibodyAnti-MPP7 antibody |
Information sourced from Uniprot.org