Anti-P4HB antibody (409-508) [S1MR] (STJ11102971)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal P4HB (409-508) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S1MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | P4HB |
| Gene ID | 5034 |
| Uniprot ID | PDIA1_HUMAN |
| Immunogen Region | 409-508 |
| Immunogen Sequence | KLGETYKDHENIVIAKMDST ANEVEAVKVHSFPTLKFFPA SADRTVIDYNGERTLDGFKK FLESGGQDGAGDDDDLEDLE EAEEPDMEEDDDQKAVKDEL |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 409-508 of human P4HB (P07237). |
Additional Info
| Post Translational Modifications | Phosphorylation of Ser-357 by FAM20C is induced by endoplasmic reticulum stress and results in a functional switch from oxidoreductase to molecular chaperone. It also promotes interaction with ERN1. |
| Function | This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations and following phosphorylation by FAM20C, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts as a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Receptor for LGALS9.the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration. |
| Protein Name | Protein Disulfide-IsomerasePdiCellular Thyroid Hormone-Binding ProteinProlyl 4-Hydroxylase Subunit BetaP55 |
| Database Links | Reactome: R-HSA-1650814Reactome: R-HSA-264876Reactome: R-HSA-3299685Reactome: R-HSA-381426Reactome: R-HSA-5358346Reactome: R-HSA-8866423Reactome: R-HSA-8957275Reactome: R-HSA-8963888Reactome: R-HSA-8964041Reactome: R-HSA-9020591Reactome: R-HSA-9020933 |
| Cellular Localisation | Endoplasmic ReticulumEndoplasmic Reticulum LumenMelanosomeCell MembranePeripheral Membrane ProteinHighly AbundantIn Some Cell TypesSeems To Be Also Secreted Or Associated With The Plasma MembraneWhere It Undergoes Constant Shedding And Replacement From Intracellular Sources (Probable)Localizes Near Cd4-Enriched Regions On Lymphoid Cell SurfacesIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage IvColocalizes With Mttp In The Endoplasmic Reticulum |
| Alternative Antibody Names | Anti-Protein Disulfide-Isomerase antibodyAnti-Pdi antibodyAnti-Cellular Thyroid Hormone-Binding Protein antibodyAnti-Prolyl 4-Hydroxylase Subunit Beta antibodyAnti-P55 antibodyAnti-P4HB antibodyAnti-ERBA2L antibodyAnti-PDI antibodyAnti-PDIA1 antibodyAnti-PO4DB antibody |
Information sourced from Uniprot.org