• Immunohistochemistry analysis of paraffin-embedded human placenta using P4HB Rabbit monoclonal antibody (STJ11102971) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using P4HB antibody (STJ11102971) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of extracts of various cell lines, using P4HB antibody (STJ11102971) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-P4HB antibody (409-508) [S1MR] (STJ11102971)

SKU:
STJ11102971

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-P4HB (409-508) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: P4HB
Gene ID: 5034
Uniprot ID: PDIA1_HUMAN
Immunogen Region: 409-508
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 409-508 of human P4HB (P07237).
Immunogen Sequence: KLGETYKDHENIVIAKMDST ANEVEAVKVHSFPTLKFFPA SADRTVIDYNGERTLDGFKK FLESGGQDGAGDDDDLEDLE EAEEPDMEEDDDQKAVKDEL
Post Translational Modifications Phosphorylation of Ser-357 by FAM20C is induced by endoplasmic reticulum stress and results in a functional switch from oxidoreductase to molecular chaperone. It also promotes interaction with ERN1.
Function This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations and following phosphorylation by FAM20C, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts as a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Receptor for LGALS9.the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration.
Protein Name Protein Disulfide-Isomerase
Pdi
Cellular Thyroid Hormone-Binding Protein
Prolyl 4-Hydroxylase Subunit Beta
P55
Database Links Reactome: R-HSA-1650814
Reactome: R-HSA-264876
Reactome: R-HSA-3299685
Reactome: R-HSA-381426
Reactome: R-HSA-5358346
Reactome: R-HSA-8866423
Reactome: R-HSA-8957275
Reactome: R-HSA-8963888
Reactome: R-HSA-8964041
Reactome: R-HSA-9020591
Reactome: R-HSA-9020933
Cellular Localisation Endoplasmic Reticulum
Endoplasmic Reticulum Lumen
Melanosome
Cell Membrane
Peripheral Membrane Protein
Highly Abundant
In Some Cell Types
Seems To Be Also Secreted Or Associated With The Plasma Membrane
Where It Undergoes Constant Shedding And Replacement From Intracellular Sources (Probable)
Localizes Near Cd4-Enriched Regions On Lymphoid Cell Surfaces
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Colocalizes With Mttp In The Endoplasmic Reticulum
Alternative Antibody Names Anti-Protein Disulfide-Isomerase antibody
Anti-Pdi antibody
Anti-Cellular Thyroid Hormone-Binding Protein antibody
Anti-Prolyl 4-Hydroxylase Subunit Beta antibody
Anti-P55 antibody
Anti-P4HB antibody
Anti-ERBA2L antibody
Anti-PDI antibody
Anti-PDIA1 antibody
Anti-PO4DB antibody

Information sourced from Uniprot.org

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