Anti-P4HA1 antibody (Internal) (STJ71115)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-P4HA1 (Internal) is suitable for use in ELISA, Immunofluorescence and Immunohistochemistry research applications. |
| Applications | Pep-ELISA/IF/IHC |
| Host | Goat |
| Reactivity | Human/Dog/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:128000.IHC: Paraffin embedded Human Spleen. Recommended concentration: 7µg/ml.IF: Strong expression of the protein seen in the ER and cytoplasm of A431 cells. Recommended concentration: 10µ |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | P4HA1 |
| Gene ID | 5033 |
| Uniprot ID | P4HA1_HUMAN |
| Accession Number | NP_000908.2; NP_001017962.1 |
| Immunogen Region | Internal |
| Immunogen Sequence | DFARKDEPDAFKE |
| Specificity | This antibody is expected to recognise both reported isoforms (NP_000908.2 and NP_001017962.1). |
Additional Info
| Function | Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. |
| Protein Name | Prolyl 4-Hydroxylase Subunit Alpha-14-Ph Alpha-1Procollagen-Proline -2-Oxoglutarate-4-Dioxygenase Subunit Alpha-1 |
| Database Links | Reactome: R-HSA-1650814 |
| Cellular Localisation | Endoplasmic Reticulum Lumen |
| Alternative Antibody Names | Anti-Prolyl 4-Hydroxylase Subunit Alpha-1 antibodyAnti-4-Ph Alpha-1 antibodyAnti-Procollagen-Proline -2-Oxoglutarate-4-Dioxygenase Subunit Alpha-1 antibodyAnti-P4HA1 antibodyAnti-P4HA antibody |
Information sourced from Uniprot.org