• STJ71115 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing endoplasmic reticulum and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ71115 (7µg/ml) staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
  • STJ71115 Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.

Anti-P4HA1 antibody (Internal) (STJ71115)

SKU:
STJ71115-100

£303.50
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Host: Goat
Applications: Pep-ELISA/IF/IHC
Reactivity: Human/Dog/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-P4HA1 (Internal) is suitable for use in ELISA, Immunofluorescence and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IHC-7µg/ml
IF-Strong expression of the protein seen in the ER and cytoplasm of A431 cells. 10µg/ml
ELISA-antibody detection limit dilution 1:128000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: P4HA1
Gene ID: 5033
Uniprot ID: P4HA1_HUMAN
Immunogen Region: Internal
Accession Number: NP_000908.2; NP_001017962.1
Specificity: This antibody is expected to recognise both reported isoforms (NP_000908.2 and NP_001017962.1).
Immunogen Sequence: DFARKDEPDAFKE
Function Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.
Protein Name Prolyl 4-Hydroxylase Subunit Alpha-1
4-Ph Alpha-1
Procollagen-Proline -2-Oxoglutarate-4-Dioxygenase Subunit Alpha-1
Database Links Reactome: R-HSA-1650814
Cellular Localisation Endoplasmic Reticulum Lumen
Alternative Antibody Names Anti-Prolyl 4-Hydroxylase Subunit Alpha-1 antibody
Anti-4-Ph Alpha-1 antibody
Anti-Procollagen-Proline -2-Oxoglutarate-4-Dioxygenase Subunit Alpha-1 antibody
Anti-P4HA1 antibody
Anti-P4HA antibody

Information sourced from Uniprot.org

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