• STJ72451 (1µg/ml) staining of Daudi cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ72451 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ72451 Immunofluorescence analysis of paraformaldehyde fixed RAW264. 7 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-p47phox-Mouse antibody (Internal) (STJ72451)

SKU:
STJ72451-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IF
Reactivity: Human/Mouse/Rat/Dog/Pig/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-p47phox-Mouse (Internal) is suitable for use in ELISA, Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IF-Immunofluorescence:Expression of the protein seen in the cytoplasm and plasma membrane of NIH3T3 and RAW264.7 cells. 10µg/ml
ELISA-antibody detection limit dilution 1:2000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: NCF1
Gene ID: 653361
Uniprot ID: NCF1_HUMAN
Immunogen Region: Internal
Accession Number: NP_035006.3
Immunogen Sequence: DEAEDPDPNYAGEP
Post Translational Modifications Phosphorylated by PRKCD.phosphorylation induces activation of NCF1 and NADPH oxidase activity.
Function NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
Protein Name Neutrophil Cytosol Factor 1
Ncf-1
47 Kda Autosomal Chronic Granulomatous Disease Protein
47 Kda Neutrophil Oxidase Factor
Ncf-47k
Neutrophil Nadph Oxidase Factor 1
Nox Organizer 2
Nox-Organizing Protein 2
Sh3 And Px Domain-Containing Protein 1a
P47-Phox
Database Links Reactome: R-HSA-1222556
Reactome: R-HSA-1236973
Reactome: R-HSA-3299685
Reactome: R-HSA-4420097
Reactome: R-HSA-5668599
Reactome: R-HSA-9013149
Reactome: R-HSA-9013404
Reactome: R-HSA-9013423
Cellular Localisation Cytoplasm
Cytosol
Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Alternative Antibody Names Anti-Neutrophil Cytosol Factor 1 antibody
Anti-Ncf-1 antibody
Anti-47 Kda Autosomal Chronic Granulomatous Disease Protein antibody
Anti-47 Kda Neutrophil Oxidase Factor antibody
Anti-Ncf-47k antibody
Anti-Neutrophil Nadph Oxidase Factor 1 antibody
Anti-Nox Organizer 2 antibody
Anti-Nox-Organizing Protein 2 antibody
Anti-Sh3 And Px Domain-Containing Protein 1a antibody
Anti-P47-Phox antibody
Anti-NCF1 antibody
Anti-NOXO2 antibody
Anti-SH3PXD1A antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance