• Western blot analysis of extracts of various cell lines, using P2RY14 antibody (STJ110477) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded rat ovary using P2RY14 Rabbit polyclonal antibody (STJ110477) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat ovary using P2RY14 rabbit polyclonal antibody (STJ110477) at dilution of 1:200 (40x lens).
  • Western blot analysis of K-562, using P2RY14 Rabbit polyclonal antibody (STJ110477) at 1:1500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-P2RY14 antibody (209-338) (STJ110477)

SKU:
STJ110477

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-P2RY14 (209-338) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: P2RY14
Gene ID: 9934
Uniprot ID: P2Y14_HUMAN
Immunogen Region: 209-338
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 209-338 of human P2RY14 (NP_055694.3).
Immunogen Sequence: ITKKIFKSHLKSSRNSTSVK KKSSRNIFSIVFVFFVCFVP YHIARIPYTKSQTEAHYSCQ SKEILRYMKEFTLLLSAANV CLDPIIYFFLCQPFREILCK KLHIPLKAQNDLDISRIKRG NTTLESTDTL
Tissue Specificity Highest expression in the placenta, adipose tissue, stomach and intestine, intermediate levels in the brain, spleen, lung and heart, lowest levels in the kidney.
Function Receptor for UDP-glucose and other UDP-sugar coupled to G-proteins. Not activated by ATP, ADP, UTP or ATP.
Protein Name P2y Purinoceptor 14
P2y14
G-Protein Coupled Receptor 105
Udp-Glucose Receptor
Database Links Reactome: R-HSA-417957
Reactome: R-HSA-418594
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-P2y Purinoceptor 14 antibody
Anti-P2y14 antibody
Anti-G-Protein Coupled Receptor 105 antibody
Anti-Udp-Glucose Receptor antibody
Anti-P2RY14 antibody
Anti-GPR105 antibody
Anti-KIAA0001 antibody

Information sourced from Uniprot.org

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