Anti-P2RX7 antibody (47-595) (STJ112534)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal P2RX7 (47-595) antibody for use in WB and ELISA in mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | P2RX7 |
| Gene ID | 5027 |
| Uniprot ID | P2RX7_HUMAN |
| Immunogen Region | 47-595 |
| Immunogen Sequence | SDKLYQRKEPVISSVHTKVK GIAEVKEEIVENGVKKLVHS VFDTADYTFPLQGNSFFVMT NFLKTEGQEQRLCPEYPTRR TLCSSDRGCKKGWMDPQSKG IQTGRCVVYEGNQKTCEVSA WCPIEAVEEAPRPALLNSAE NFTVLIKNNIDFPGHNYTTR NILPGLNITCTFHKTQNPQC PIFRLGDIFRETGDNFSDVA IQGGIMGIEIYWDCNLDRWF HHCRPKYSFRRLDDKTTNV |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 47-334 of human P2RX7 (NP_002553.3). |
Additional Info
| Tissue Specificity | Widely expressed with highest levels in brain and immune tissues. Isoform B: Predominant form in many tissues. |
| Post Translational Modifications | Phosphorylation results in its inactivation. ADP-ribosylation at Arg-125 is necessary and sufficient to activate P2RX7 and gate the channel. Palmitoylation of several cysteines in the C-terminal cytoplasmic tail is required for efficient localization to cell surface. Palmitoylation prevents channel desensitization by physically anchoring the palmitoylated groups to the membrane. |
| Function | ATP-gated nonselective transmembrane cation channel that requires high millimolar concentrations of ATP for activation. Upon ATP binding, it rapidly opens to allow the influx of small cations Na(+) and Ca(2+), and the K(+) efflux. Also has the ability to form a large pore in the cell membrane, allowing the passage of large cationic molecules. In microglia, may mediate NADPH transport across the plasma membrane. In immune cells, P2RX7 acts as a molecular sensor in pathological inflammatory states by detecting and responding to high local concentrations of extracellar ATP. In microglial cells, P2RX7 activation leads to the release of pro-inflammatory cytokines, such as IL-1beta and IL-18, through the activation of the NLRP3 inflammasome and caspase-1. Cooperates with KCNK6 to activate NLRP3 inflammasome. Activates death pathways leading to apoptosis and autophagy. Activates death pathways leading to pyroptosis. Isoform B: Shows ion channel activity but no macropore function. Isoform H: Non-functional channel. Isoform J: Non-functional channel. |
| Protein Name | P2x Purinoceptor 7P2x7Atp ReceptorP2z ReceptorPurinergic Receptor |
| Database Links | Reactome: R-HSA-139853Reactome: R-HSA-418346Reactome: R-HSA-844456Reactome: R-HSA-9660826Reactome: R-HSA-9856532 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-P2x Purinoceptor 7 antibodyAnti-P2x7 antibodyAnti-Atp Receptor antibodyAnti-P2z Receptor antibodyAnti-Purinergic Receptor antibodyAnti-P2RX7 antibody |
Information sourced from Uniprot.org