• STJ72755 (0. 3µg/ml) staining of A549 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ72755 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).

Anti-P2rx4-Mouse antibody (Internal) (STJ72755)

SKU:
STJ72755-100

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Host: Goat
Applications: Pep-ELISA/WB/IF/IHC
Reactivity: Human/Mouse/Rat/Dog/Pig/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-P2rx4-Mouse (Internal) is suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.3-1µg/ml
IHC-5µg/ml
IF-Strong expression of the protein seen in the cytoplasm of A431 cells. 10µg/ml
ELISA-antibody detection limit dilution 1:16000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: P2RX4
Gene ID: 5025
Uniprot ID: P2RX4_HUMAN
Immunogen Region: Internal
Accession Number: NP_035156.2
Immunogen Sequence: ETDSVVSSVTTKAK
Protein Name P2x Purinoceptor 4
P2x4
Atp Receptor
Purinergic Receptor
Database Links Reactome: R-HSA-139853
Reactome: R-HSA-418346
Reactome: R-HSA-9660826
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Lysosome Membrane
Alternative Antibody Names Anti-P2x Purinoceptor 4 antibody
Anti-P2x4 antibody
Anti-Atp Receptor antibody
Anti-Purinergic Receptor antibody
Anti-P2RX4 antibody

Information sourced from Uniprot.org

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