• Western blot analysis of extracts of various cell lines, using OTX2 antibody (STJ27428) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 60s.

Anti-OTX2 antibody (140-280) (STJ27428)

SKU:
STJ27428

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Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-OTX2 (140-280) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: OTX2
Gene ID: 5015
Uniprot ID: OTX2_HUMAN
Immunogen Region: 140-280
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 140-280 of human OTX2 (NP_068374.1).
Immunogen Sequence: TPPSSTSVPTIASSSAPVSI WSPASISPLSDPLSTSSSCM QRSYPMTYTQASGYSQGYAG STSYFGGMDCGSYLTPMHHQ LPGPGATLSPMGTNAVTSHL NQSPASLSTQGYGASSLGFN STTDCLDYKDQTASWKLNFN A
Function Transcription factor probably involved in the development of the brain and the sense organs. Can bind to the bicoid/BCD target sequence (BTS): 5'-TCTAATCCC-3'.
Protein Name Homeobox Protein Otx2
Orthodenticle Homolog 2
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Homeobox Protein Otx2 antibody
Anti-Orthodenticle Homolog 2 antibody
Anti-OTX2 antibody

Information sourced from Uniprot.org

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